Determination the content of cysteine in food by potassium dichromate spectrophotometry

Xin-rong Wen, C. Tu
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Abstract

In this paper, a new method for the determination of cysteine by potassium dichromate spectrophotometry is established. In the H2SO4 medium, potassium dichromate has strong oxidation. Potassium dichromate can react with the hydrosulfuryl(-SH) in cysteine, which makes potassium dichromate fade, and the fading degree of potassium dichromate is proportional to the amount of cysteine added. Therefore, the content of cysteine can be quantitatively determined by determinating the decrease value of absorbance. The various influencing factors for the determination the content of cysteine by potassium bichromate spectrophotometry are investigated. Under the optimal conditions, the content of cysteine in food is determined, and the results are satisfactory.
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重铬酸钾分光光度法测定食品中半胱氨酸含量
本文建立了重铬酸钾分光光度法测定半胱氨酸的新方法。重铬酸钾在H2SO4介质中具有很强的氧化性。重铬酸钾能与半胱氨酸中的氢硫酰(-SH)发生反应,使重铬酸钾褪色,重铬酸钾的褪色程度与半胱氨酸的加入量成正比。因此,可以通过测定吸光度降低值来定量测定半胱氨酸的含量。研究了重铬酸钾分光光度法测定半胱氨酸含量的各种影响因素。在此条件下,对食品中半胱氨酸的含量进行了测定,结果令人满意。
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