Pub Date : 2023-10-01DOI: 10.1051/bioconf/20235603010
Catarina P. Gomes, Miguel Cachão, Ana Teresa Batista, Ana Margarida Chambel, Maria do Amparo Godinho, L. Mendes
In current days, the major challenges for farmers are the impact of plant protection products (PPP) on the public health, the environment protection, residues reduction, bees and non-target organisms, as well as, the withdrawal of many active ingredients and climate changes. Given the current situation, sustainable use of PPP is a main objective and priority. The decisions making of using PPP of 235 winegrowers from Palmela region, which do not have regular technical assistance, were assessed during 2016 until 2019. The data analysed included the number of applications, the dosages used and the compliance of pre-harvest interval (PHI). For each year, it was observed that a winegrower, on average, made seven treatments, although the tendency is a decrease to reduce the number of treatments. The PPP most used belong to the groups 3 (Triazoles), M02 (Inorganic) and M04 + 4 (Phthalimides + Phenyl Amides) according FRAC Code. Regardless of the climatic conditions and the disease pressure in the vineyard, the winegrowers used PPP every 14 days. Fear and “empiric experience” sometimes overtake knowledge and technology. That could only be changed with trust between technical assistance and farmers. That could be the solution to face the mentioned challenges and to offer sustainable wines from Palmela region.
{"title":"The behaviour of grapevine growers in the decision-making of using Plant Protection Products (PPP) from Palmela region","authors":"Catarina P. Gomes, Miguel Cachão, Ana Teresa Batista, Ana Margarida Chambel, Maria do Amparo Godinho, L. Mendes","doi":"10.1051/bioconf/20235603010","DOIUrl":"https://doi.org/10.1051/bioconf/20235603010","url":null,"abstract":"In current days, the major challenges for farmers are the impact of plant protection products (PPP) on the public health, the environment protection, residues reduction, bees and non-target organisms, as well as, the withdrawal of many active ingredients and climate changes. Given the current situation, sustainable use of PPP is a main objective and priority. The decisions making of using PPP of 235 winegrowers from Palmela region, which do not have regular technical assistance, were assessed during 2016 until 2019. The data analysed included the number of applications, the dosages used and the compliance of pre-harvest interval (PHI). For each year, it was observed that a winegrower, on average, made seven treatments, although the tendency is a decrease to reduce the number of treatments. The PPP most used belong to the groups 3 (Triazoles), M02 (Inorganic) and M04 + 4 (Phthalimides + Phenyl Amides) according FRAC Code. Regardless of the climatic conditions and the disease pressure in the vineyard, the winegrowers used PPP every 14 days. Fear and “empiric experience” sometimes overtake knowledge and technology. That could only be changed with trust between technical assistance and farmers. That could be the solution to face the mentioned challenges and to offer sustainable wines from Palmela region.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87489724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236505027
R. Kattakhanova, U. N. Kanataeva
In order to clarify the role of the prostacyclin-thromboxane system (PTS) in the development of acute circulatory disorders in the early postoperative period after coronary artery bypass surgery, its condition was studied in 86 patients with coronary artery disease. In patients with the development of vascular insufficiency and shock, a decrease in the level of thromboxane in the blood was revealed, while in patients with arterial hypertension, elevated concentrations of thromboxane in the blood were determined in the absence of changes in the level of prostacyclin. There were no significant differences in the content of prostacyclin and thromboxane between groups of patients with uncomplicated postoperative course and with the development of such complications as heart failure and hypovolemia.
{"title":"The state of the prostacyclin-thromboxane system in patients after aorto-coronary bypass surgery","authors":"R. Kattakhanova, U. N. Kanataeva","doi":"10.1051/bioconf/20236505027","DOIUrl":"https://doi.org/10.1051/bioconf/20236505027","url":null,"abstract":"In order to clarify the role of the prostacyclin-thromboxane system (PTS) in the development of acute circulatory disorders in the early postoperative period after coronary artery bypass surgery, its condition was studied in 86 patients with coronary artery disease. In patients with the development of vascular insufficiency and shock, a decrease in the level of thromboxane in the blood was revealed, while in patients with arterial hypertension, elevated concentrations of thromboxane in the blood were determined in the absence of changes in the level of prostacyclin. There were no significant differences in the content of prostacyclin and thromboxane between groups of patients with uncomplicated postoperative course and with the development of such complications as heart failure and hypovolemia.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73640830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236303016
V. B. Leushin, R. Yusupov, A. G. Isaicheva
Modern train interval control systems contain communication channels between traffic organizers and the train locomotive, using rail and inductive rail as communication lines. The rail threads that make up these lines are an integral part of the traction network and, therefore, the traction current is channeled through them. Bearing this in mind, the signals of these channels operate under the conditions of strong interference from traction current. At present, continuous automatic locomotive signalling systems continue to improve, so it is necessary to have information on the relationship between the immunity of their channels, in particular under conditions of traction current interference, from different signal parameters, such as carrier frequency. This article deals with the method of evaluating the influence of the frequency of the carrier signal on the noise resistance of the channels comprising rail and inductive rail lines.
{"title":"Evaluation of Noise Immunity of Channels with Rail and Inductive-Rail Lines","authors":"V. B. Leushin, R. Yusupov, A. G. Isaicheva","doi":"10.1051/bioconf/20236303016","DOIUrl":"https://doi.org/10.1051/bioconf/20236303016","url":null,"abstract":"Modern train interval control systems contain communication channels between traffic organizers and the train locomotive, using rail and inductive rail as communication lines. The rail threads that make up these lines are an integral part of the traction network and, therefore, the traction current is channeled through them. Bearing this in mind, the signals of these channels operate under the conditions of strong interference from traction current. At present, continuous automatic locomotive signalling systems continue to improve, so it is necessary to have information on the relationship between the immunity of their channels, in particular under conditions of traction current interference, from different signal parameters, such as carrier frequency. This article deals with the method of evaluating the influence of the frequency of the carrier signal on the noise resistance of the channels comprising rail and inductive rail lines.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75832619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236505010
F.S. Saidvaliev, M.M. Mavlonov
The article is aimed to determine the specific features of common neurological diseases, migraine and epilepsy comorbidity, correct selection of drugs and increasing the effectiveness of treatment in cases where migraine and epilepsy are comorbid. A total of 60 (100%) patients were recruited during the study. 30 of them (the main group) were diagnosed with migraine and epilepsy and were prescribed lamotrigine, and 30 patients (the control group) were diagnosed with migraine and epilepsy and were prescribed convulex. The clinical course of migraine, the level of cognitive disorders, the quality of life of patients, and the level of limitation of daily work were studied in patients of both groups.
{"title":"Improving the treatment of migraine and epilepsy comorbidity","authors":"F.S. Saidvaliev, M.M. Mavlonov","doi":"10.1051/bioconf/20236505010","DOIUrl":"https://doi.org/10.1051/bioconf/20236505010","url":null,"abstract":"The article is aimed to determine the specific features of common neurological diseases, migraine and epilepsy comorbidity, correct selection of drugs and increasing the effectiveness of treatment in cases where migraine and epilepsy are comorbid. A total of 60 (100%) patients were recruited during the study. 30 of them (the main group) were diagnosed with migraine and epilepsy and were prescribed lamotrigine, and 30 patients (the control group) were diagnosed with migraine and epilepsy and were prescribed convulex. The clinical course of migraine, the level of cognitive disorders, the quality of life of patients, and the level of limitation of daily work were studied in patients of both groups.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74742012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236101013
Xiaochuan Xu
Liposomes, as biocompatible and safe nanocarriers with easily modified surfaces, can be well used in the field of antitumor. Their targeting properties have contributed to the reduction of drug dosage and non-target cell toxicity. To further exploit the targeting properties of liposomes, passive targeting liposomes, active targeting liposomes and physicochemical targeting liposomes have been constructed by surface modification. This paper summarizes the mechanisms of action of different types of targeted liposomes and describes the specific role of liposomes in overcoming tumor resistance, immunotherapy and helping drugs to cross the blood-brain barrier, and summarizes the current development issues and future directions.
{"title":"Advances in liposome research in the field of antitumor","authors":"Xiaochuan Xu","doi":"10.1051/bioconf/20236101013","DOIUrl":"https://doi.org/10.1051/bioconf/20236101013","url":null,"abstract":"Liposomes, as biocompatible and safe nanocarriers with easily modified surfaces, can be well used in the field of antitumor. Their targeting properties have contributed to the reduction of drug dosage and non-target cell toxicity. To further exploit the targeting properties of liposomes, passive targeting liposomes, active targeting liposomes and physicochemical targeting liposomes have been constructed by surface modification. This paper summarizes the mechanisms of action of different types of targeted liposomes and describes the specific role of liposomes in overcoming tumor resistance, immunotherapy and helping drugs to cross the blood-brain barrier, and summarizes the current development issues and future directions.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74866465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20235902002
Rui Zhang
The prevention and diagnosis of lung cancer in the Chinese population has received extensive attention in past few years. Many studies have suggested that genetic predisposition may be a risk factor for lung cancer and an important means for early diagnosis of carcinoma of the lung. At present, the research on the molecular mechanism of lung cancer has been carried out on the whole genome, and a large number of SNPs have also been found in the Chinese population, these results suggest that the genetic predisposing factors of lung cancer in Chinese population are different from those in foreign population.
{"title":"Meta-analysis of lung cancer in Chinese population in genetic predisposition","authors":"Rui Zhang","doi":"10.1051/bioconf/20235902002","DOIUrl":"https://doi.org/10.1051/bioconf/20235902002","url":null,"abstract":"The prevention and diagnosis of lung cancer in the Chinese population has received extensive attention in past few years. Many studies have suggested that genetic predisposition may be a risk factor for lung cancer and an important means for early diagnosis of carcinoma of the lung. At present, the research on the molecular mechanism of lung cancer has been carried out on the whole genome, and a large number of SNPs have also been found in the Chinese population, these results suggest that the genetic predisposing factors of lung cancer in Chinese population are different from those in foreign population.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73026515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20235801014
Mariana Galabova, N. Stoyanov, P. Mitev
Analysis of the chemical composition of volatile substances in two distillates from Prunus domestica “Stanley” cultivated in the village of Rumyantsevo, Lovech, Bulgaria. Production is in an industrial environment, fermentation and distillation are carried out under equal conditions, but on different starting materials. One batch PDS-1 distilled from fermented mash and second batch PDS-2 distilled from fermented juice. The distillation apparatus is equipped with a rectifying column with 4 trays, as well as a water reflux condenser. Determination of congeners in both samples was done by gas chromatography principle OIV-MA-BS-14:2009. Methodology used for measuring alcohol content, cyanide derivatives, furfural and total acidity is according to the requirements of the OIV. In PDS-1 methanol (1094.7 g/hL a.a.) is almost 8% higher than in PDS-2 (1015.8 g/hL a.a.), it was found insignificant increase of furfural content in distillates of PDS-1 than in PDS-2. Concerning esters, aldehydes and higher alcohols the amount in PDS- 1 is significantly higher than in PDS-2, respectively 203.06 g/hL, 14.2 g/hL and 484.6 g/hL in batch fermented with hard particles, and 14.2 g/hL, 8.6 g/hL, 267.9 g/hL in batch produced from fermented juice.
{"title":"Comparison between the volatile substances in spirits beverages distilled by two different initial materials of Prunus domestica “Stanley” in an industrial environment","authors":"Mariana Galabova, N. Stoyanov, P. Mitev","doi":"10.1051/bioconf/20235801014","DOIUrl":"https://doi.org/10.1051/bioconf/20235801014","url":null,"abstract":"Analysis of the chemical composition of volatile substances in two distillates from Prunus domestica “Stanley” cultivated in the village of Rumyantsevo, Lovech, Bulgaria. Production is in an industrial environment, fermentation and distillation are carried out under equal conditions, but on different starting materials. One batch PDS-1 distilled from fermented mash and second batch PDS-2 distilled from fermented juice. The distillation apparatus is equipped with a rectifying column with 4 trays, as well as a water reflux condenser. Determination of congeners in both samples was done by gas chromatography principle OIV-MA-BS-14:2009. Methodology used for measuring alcohol content, cyanide derivatives, furfural and total acidity is according to the requirements of the OIV. In PDS-1 methanol (1094.7 g/hL a.a.) is almost 8% higher than in PDS-2 (1015.8 g/hL a.a.), it was found insignificant increase of furfural content in distillates of PDS-1 than in PDS-2. Concerning esters, aldehydes and higher alcohols the amount in PDS- 1 is significantly higher than in PDS-2, respectively 203.06 g/hL, 14.2 g/hL and 484.6 g/hL in batch fermented with hard particles, and 14.2 g/hL, 8.6 g/hL, 267.9 g/hL in batch produced from fermented juice.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73248082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236501007
N. Ergasheva
This paper reports on the effect of seedling thickness on stem height and leaf number of oilseed sunflower. According to him, the local Dilbar (st) variety compared to foreign Buzuluk and Rodnik varieties, when the seedling thickness was set at 50, 60, and 70,000 per hectare, the plant was superior in terms of height and number of leaves, but when the number of seedlings was increased to 80,000, the growth slowed down and reached an average of 226.4 cm. and while the height of the plant in the Buzuluk variety is average, it was determined that the height of the plant in the Rodnik variety is high, i.e. 234.3 cm, at the thickness of 80,000 seedlings per hectare.
{"title":"Effect of seedling thickness on stem height and number of leaves of oil sunflower cultivars","authors":"N. Ergasheva","doi":"10.1051/bioconf/20236501007","DOIUrl":"https://doi.org/10.1051/bioconf/20236501007","url":null,"abstract":"This paper reports on the effect of seedling thickness on stem height and leaf number of oilseed sunflower. According to him, the local Dilbar (st) variety compared to foreign Buzuluk and Rodnik varieties, when the seedling thickness was set at 50, 60, and 70,000 per hectare, the plant was superior in terms of height and number of leaves, but when the number of seedlings was increased to 80,000, the growth slowed down and reached an average of 226.4 cm. and while the height of the plant in the Buzuluk variety is average, it was determined that the height of the plant in the Rodnik variety is high, i.e. 234.3 cm, at the thickness of 80,000 seedlings per hectare.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73850934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236604001
D. Bogoutdinov, Ilya Mityushev, Natalya Girsova, Tatyana Kastalyeva, Nelly Chigina
The European lantern fly, Dictyophara europaea (Linnaeus, 1767) (Insecta: Hemiptera: Dictyopharidae) is an abundant species in Eurasia and North Africa, inhabiting the grassy vegetation of open spaces, and does not have the status of a pest of agricultural plants. The purpose of this study was to assess the relative prevalence of planthopper under conditions of eco- and agroecosystems on herbaceous and woody plants in the Samara region of Russia for the period 1992-2016. Along with natural vegetation, D. europaea was also registered on potatoes, barley and perennial fodder grasses, as well as on cherry, plum and black poplar. The feeding of D. europaea was observed on potatoes, watermelon, pumpkin, alfalfa, as well as on wild plants: steppe sage, tuberous rank, chicory, and on woody plants: common lilac and bird cherry. It was found that the abundance of D. europaea increased by 2.2 times in the 21st century compared to the 20th century. It is reported that under conditions of the Samara region of Russia, D. europaea can be a potential vector of phytoplasma belonging to the pigeon pea witches’-broom group – 16SrIX, which may have phytosanitary significance for cultivated plants.
{"title":"To the biology of the European lantern fly, Dictyophara europaea, in the Middle Volga region of Russia","authors":"D. Bogoutdinov, Ilya Mityushev, Natalya Girsova, Tatyana Kastalyeva, Nelly Chigina","doi":"10.1051/bioconf/20236604001","DOIUrl":"https://doi.org/10.1051/bioconf/20236604001","url":null,"abstract":"The European lantern fly, Dictyophara europaea (Linnaeus, 1767) (Insecta: Hemiptera: Dictyopharidae) is an abundant species in Eurasia and North Africa, inhabiting the grassy vegetation of open spaces, and does not have the status of a pest of agricultural plants. The purpose of this study was to assess the relative prevalence of planthopper under conditions of eco- and agroecosystems on herbaceous and woody plants in the Samara region of Russia for the period 1992-2016. Along with natural vegetation, D. europaea was also registered on potatoes, barley and perennial fodder grasses, as well as on cherry, plum and black poplar. The feeding of D. europaea was observed on potatoes, watermelon, pumpkin, alfalfa, as well as on wild plants: steppe sage, tuberous rank, chicory, and on woody plants: common lilac and bird cherry. It was found that the abundance of D. europaea increased by 2.2 times in the 21st century compared to the 20th century. It is reported that under conditions of the Samara region of Russia, D. europaea can be a potential vector of phytoplasma belonging to the pigeon pea witches’-broom group – 16SrIX, which may have phytosanitary significance for cultivated plants.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74163638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20235703003
V. Nazarov, E. Semenova, E. Andronova, I. Tsokalo, Elena Semenova
The features of clonal micropropagation in three variations of Ludisia discolor were studied. Brood buds, apical shoot meristems, immature seeds and mature seeds were used as explants. They are grown using Murashige & Skoog modified medium. A special technique has been developed for the sterilization and planting of microscopic mature seeds. Mature seeds germinated for 60-70 days. Seeds from 25-day-old capsules showed the best results among immature seeds. They gave mass shoots already on the 25-30 day after planting. The roller of the future first leaf of the protocorms was laid in the dark 2 months after germination. Switching to the light mode induced rapid gemmorizogenesis - the formation of the first green leaves, stem and adventitious roots. Seeds from 15-day-old capsules did not germinate at all. Planting with immature seeds is the most effective method of clonal reproduction of L. discolor in vitro.
{"title":"Development of Clonal Micropropagation Technology for Ludisia Discolor (Ker Gawl.) A. Rich. In Vitro Conditions","authors":"V. Nazarov, E. Semenova, E. Andronova, I. Tsokalo, Elena Semenova","doi":"10.1051/bioconf/20235703003","DOIUrl":"https://doi.org/10.1051/bioconf/20235703003","url":null,"abstract":"The features of clonal micropropagation in three variations of Ludisia discolor were studied. Brood buds, apical shoot meristems, immature seeds and mature seeds were used as explants. They are grown using Murashige & Skoog modified medium. A special technique has been developed for the sterilization and planting of microscopic mature seeds. Mature seeds germinated for 60-70 days. Seeds from 25-day-old capsules showed the best results among immature seeds. They gave mass shoots already on the 25-30 day after planting. The roller of the future first leaf of the protocorms was laid in the dark 2 months after germination. Switching to the light mode induced rapid gemmorizogenesis - the formation of the first green leaves, stem and adventitious roots. Seeds from 15-day-old capsules did not germinate at all. Planting with immature seeds is the most effective method of clonal reproduction of L. discolor in vitro.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78411573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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