Antioxidant activity of methanol extract of cassava leaves (Manihot esculenta Crantz) using CUPRAC method

Abstract

Background: Antioxidants are a group of organic molecules and enzymes that work synergistically to enhance cellular defenses and combat oxidative stress. Antioxidants can come from plants because they contain many groups of phytochemical compounds and vitamins. One of the plants that has compounds with antioxidant activity is cassava. Objective: To measure the antioxidant activity of methanol extract of cassava leaves using the CUPRAC method. Methods: Cassava leaves were extracted by maceration using methanol as a solvent with the addition of 5% H2SO4. Phytochemical screening of flavonoids with Mg and HCl, while phenol with the addition of FeCl3. The antioxidant activity based on the reaction of the test solution with CUPRAC reagent. Antioxidant activity can be determined by IC50 value by using x value as concentration and y value as absorbance. Results: The results of phytochemical screening showed that the methanol extract of cassava leaves contained flavonoids and phenolic groups. The antioxidant capacity of the methanol extract of cassava leaves obtained an IC50 value of 156.03 ppm, while quercetin has a very strong capacity as an antioxidant with an IC50 of 9.83 ppm. Conclusion: The methanol extract of cassava leaves has a weak antioxidant capacity in reducing metal ions. Keywords: Cassava leaves, Manihot esculenta Crantz, methanol extract, antioxidants, CUPRAC.