{"title":"Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor","authors":"Zhuqing Wang, Jinhua Li, M. Kimura, T. Ono","doi":"10.1109/NANO.2016.7751536","DOIUrl":null,"url":null,"abstract":"Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.","PeriodicalId":6646,"journal":{"name":"2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO)","volume":"32 1","pages":"681-682"},"PeriodicalIF":0.0000,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NANO.2016.7751536","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.
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