Characterization of Human Umbilical Cord Mesenchymal Stem Cells-Derived Conditioned Medium

Abstract

Medium where the stem cells are cultured is called conditioned medium (CM). In CM, Mesenchymal Stem Cells (MSCs) secrete different growth factors and cytokines with anti-inflammatory and anti-fibrotic effects. Numerous questions need to be answered before MSCs-derived CM can be used for the therapy of various diseases/conditions. The purpose of the present study is to analyze Human Umbilical Cord MSCs-derived CM for the presence of anti-inflammatory and regeneration-promoting cytokines. In our study of CM, immunoassay tests showed that all analyzed chemokines gave signals in the assays in positive control parallel wells. Thus all chemokines are functional and accurate to the indicated levels. Characterization of growth factors and cytokines in the MSCs-derived CM is crucial for further translation of CM in therapy of various diseases/conditions.