Clinical study of RT-PCR in children with acute respiratory viruses in Urumqi

Abstract

Objective To analyze four viruses [human adenovirus (HADV), human bocavirus (HBoV), human metapneumovirus (HMPV), human respiratory syncytial virus (HRSV) ] infection situation in children with acute lower respiratory tract infection by multiplex fluorescent reverse transcription polymerase chain reaction (RT-PCR). Methods A total of 1 045 hospitalized children with acute respiratory infections were enrolled in the specimens of deep nasopharyngeal secretions.Multiple fluorescent RT-PCR was used to detect four markers of respiratory virus, and the clinical epidemiological characteristics were analyzed in terms of virus distribution and seasonal factors. Results Of the 1 045 patients, 281 were positive for 4 respiratory viruses, and the total positive rate was 26.89%.Among them, 194(69.04%) were infected with a single virus, 87(30.96%) were infected with mixed virus, and the highest was HMPV, 120 cases (42.70%), followed by HRSV infection in 119 cases (42.35%); 229 cases (36.06%) were positive in ≤3 years old group, 114 cases (42.22%) were positive in 4-7 years old group, ≥8 years old group in each age group positive in 63 cases (45.00%). From the perspective of seasonal distribution, the detection rates of spring, summer, autumn and winter were 25.68%, 28.24%, 49.34%, and 65.57%, respectively. Conclusions Multiplex fluorescent RT-PCR can rapidly detect acute respiratory virus in children in Urumqi.The main infections of respiratory virus are HMPV and HRSV infection.The highest age of respiratory virus infection is ≥8 years old.The incidence of respiratory virus infection is high in autumn and winter period. Key words: Respiratory tract infection; Respiratory syncytial viruses; Influenza virus; Boka virus; Metapneumovirus