Electromagnet-actuated droplet platform for sample-to-answer genetic detection

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS 2013) : Taipei, Taiwan, 20-24 January 2013. IEEE International Conference on Micro Electro Mechanical Systems (26th : 2013 : Taipei, Taiwan) Pub Date : 2013-03-07 DOI:10.1109/MEMSYS.2013.6474186

C. Chiou, D. J. Shin, S. Hosmane, Y. Zhang, T. H. Wang

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Abstract

This paper presents a magnetically actuated droplet platform for automated sample preparation and gene detection. Our platform integrates nucleic acid extraction using silicacoated magnetic particles with real-time polymerase chain reaction (PCR) on a single cartridge. The device consists of seven compartments in which reagent droplets are serially loaded. The assay utilizes solid phase nucleic acid isolation chemistry in order to purify genomic DNA from crude samples and elute the material in a PCR-ready buffer. Silicacoated magnetic particle provides a mobile solid phase that can be merged with and removed from reagents efficiently using a combination of planar coils and surface topographical features. Each droplet operation was characterized and evaluated. Using our platform, automated sample processing from whole blood to PCR-ready droplet, followed by amplification was performed successfully. This work demonstrates a fully integrated genetic assay platform for simple and efficient droplet manipulation.

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用于样本到答案基因检测的电磁铁驱动液滴平台

本文介绍了一种用于自动样品制备和基因检测的磁驱动液滴平台。我们的平台集成了核酸提取使用硅涂层磁性颗粒与实时聚合酶链反应(PCR)在一个单一的墨盒。该装置由七个隔间组成，其中依次装入试剂液滴。该分析利用固相核酸分离化学，以便从粗样品中纯化基因组DNA，并在pcr准备好的缓冲液中洗脱材料。硅涂层磁颗粒提供了一种流动固相，可以使用平面线圈和表面地形特征的组合有效地与试剂合并和去除。每个液滴操作都进行了表征和评价。使用我们的平台，从全血到pcr准备滴的自动样品处理，然后进行扩增成功。这项工作展示了一个完全集成的遗传分析平台，用于简单有效的液滴操作。

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2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS 2013) : Taipei, Taiwan, 20-24 January 2013. IEEE International Conference on Micro Electro Mechanical Systems (26th : 2013 : Taipei, Taiwan)

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