Development of a method for quantitative determination of the amount of flavonoids in Artemisia scoparia herb

E. E. Airapetyan, Victoria N. Leonova, D. Konovalov
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Abstract

Plants of the genus Artemisia have a rich complex of biologically active compounds, among which essential oils, sesquiterpene lactones, flavonoids, and acetylenic compounds predominate. Particular attention is drawn to Artemisia scoparia Waldst. et Kit. Due to a wide range of therapeutic activity, wormwood paniculata is used as a choleretic, hepatoprotective, antimicrobial, anti-inflammatory agent. Flavonoids are one of the leading groups of biologically active compounds (BAS) in water and water-alcohol extracts from the aerial part of Artemisia scoparia. These compounds have powerful hepatoprotective, choleretic, anti-inflammatory, antiviral effects. Objective: development of a method for quantitative determination of the amount of flavonoids in the herb Artemisia scoparia. Materials and methods. The object of the study was Artemisia scoparia herb, collected in the flowering phase in the Nikitsky Botanical Garden in 2016-2020. Quantitative determination of the amount of flavonoids in raw materials was carried out by UV spectroscopy. Results. It has been established that the differential spectrum of the complex compound of the studied extract with aluminum chloride does not differ in terms of the position of the light absorption maximum (410 nm) from the differential spectrum of the complex of rutin with aluminum ions. The content of total flavonoids in terms of rutin in Artemisia scoparia herb was 1.95% ± 0.05. Conclusion. A method has been developed for the quantitative determination of the amount of flavonoids in the raw material of Artemisia scoparia by UV spectrophotometry in terms of rutin.
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黄花蒿中黄酮类化合物含量测定方法的建立
青蒿属植物具有丰富的生物活性化合物复合物,其中以精油、倍半萜内酯、黄酮类化合物和乙炔类化合物为主。特别值得注意的是黄花蒿。等装备。由于其广泛的治疗活性,艾草被用作一种降胆、保肝、抗菌、抗炎剂。黄酮类化合物是黄花蒿地上部水和水醇提取物中主要的生物活性化合物之一。这些化合物具有强大的保肝、降胆、抗炎、抗病毒作用。目的:建立黄酮类化合物含量的定量测定方法。材料和方法。研究对象为2016-2020年在Nikitsky植物园花期采集的黄花蒿(Artemisia scoparia)。采用紫外光谱法对原料中黄酮类化合物的含量进行了定量测定。结果。结果表明,在所研究的萃取物与氯化铝配合物的微分光谱中,芦丁与铝离子配合物的光吸收最大值(410 nm)的位置没有差别。以芦丁为单位的黄酮类化合物含量为1.95%±0.05。结论。建立了以芦丁为单位的紫外分光光度法定量测定青蒿原料中黄酮类化合物含量的方法。
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