Nondestructive testing in microfabrication using bacteria

T.J. Ferreira , A.R. Farinha , T.G. Santos , R. Miranda , C.C.C.R. Carvalho , M.T. Vieira
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引用次数: 1

Abstract

Micromanufacturing has increased rapidly at scientific, technological and industrial levels. However, this evolution has not been followed by a parallel development of Non Destructive Testing (NDT) techniques. The available techniques are, generally, unable to detect microdefects. In this work, two types of microgeometries were produced by Micro Powder Injection Molding (μPIM) of stainless steel AISI316L: specimens for tensile tests (simple geometry) and microscrews (complex geometry). During the process optimization, different injection conditions of temperature and pressure were tested, as well as various temperatures for thermal debinding and sintering. Throughout the process, detectable and undetectable defects by NDT techniques were produced, which were used in the assays to assess the role of bacteria in the detection of defects. After adding bacterial suspensions of Staphylococcus aureus or Rhodococcus erythropolis cells, the microcomponents were subjected to magnetic or electric fields to facilitate mobility of bacteria towards the defects. This new methodology to detect defects produced during microfabrication can be a good solution for inspection of microdefects.

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利用细菌进行微加工的无损检测
微制造在科学、技术和工业层面迅速发展。然而,这种演变并没有伴随着无损检测技术的并行发展。现有的技术通常无法检测微缺陷。本研究采用微粉末注射成型技术(μPIM)对AISI316L不锈钢进行了拉伸试验试样(简单几何形状)和微螺钉(复杂几何形状)两种微几何形状的制备。在工艺优化过程中,测试了不同的注射温度和压力条件,以及不同的热脱脂和烧结温度。在整个过程中,通过无损检测技术产生可检测和不可检测的缺陷,这些缺陷被用于评估细菌在缺陷检测中的作用。在加入金黄色葡萄球菌或红红球菌细胞的菌悬液后,对微组分施加磁场或电场,以促进细菌向缺陷移动。这种检测微加工缺陷的新方法为微缺陷检测提供了良好的解决方案。
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