Molecular diversity of phosphate solubilizing bacteria isolated from the rhizosphere of chickpea, mustard and wheat

Manu Solanki , B.S. Kundu , Kiran Nehra
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引用次数: 17

Abstract

Molecular diversity of native phosphate solubilizing bacteria from rhizosphere of important crops like chickpea (Cicer arietinum), mustard (Brassica campestris) and wheat (Triticium aestivum) grown in Haryana was studied A total of 193 isolates were selected from the rhizosphere of chickpea (76), mustard (68) and wheat (49) based on zone of P-solubilization. These were screened for P-solubilization on solid as well as liquid Pikovskaya's medium. All the isolates showed large variations in P-solubilization.On the basis of the 20 biochemical tests, a dendrogram was constructed using UPGMA with which all the PSB isolates were clustered in nine major groups. The isolates from chickpea, mustard and wheat rhizosphere were randomly distributed. Twenty three PSB isolates representing nine biochemical groups were selected and genetic diversity was determined by amplified ribosomal DNA restriction analysis (ARDRA) using Hinf1 and Msp1. The banding pattern was analyzed using NT-SYS programme and a dendrogram was constructed. The isolates were divided into 10 ARDRA groups at 85% similarity level. Majority of the isolates fell in one group while the remaining isolates constituted the separate groups. PSB from different crops rhizosphere were randomly distributed. Biochemical groups did not correlate to the ARDRA groups.

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鹰嘴豆、芥菜和小麦根际溶磷细菌的分子多样性
对哈里亚纳邦鹰嘴豆(Cicer arietinum)、芥菜(Brassica campestris)和小麦(Triticium aestivum)等重要作物根际天然增磷细菌的分子多样性进行了研究。根据磷增溶区,从鹰嘴豆(76)、芥菜(68)和小麦(49)根际共筛选了193株分离菌株。筛选了它们在固体和液体Pikovskaya培养基上对p的增溶作用。所有菌株的p溶解能力都有很大的差异。在20个生化指标的基础上,利用UPGMA构建了PSB分离株的树状图,并将其聚类为9个主要类群。从鹰嘴豆、芥菜和小麦根际中分离的菌株随机分布。选取9个生化类群23株PSB分离株,利用Hinf1和Msp1进行扩增核糖体DNA限制性内切分析(ARDRA),确定其遗传多样性。利用NT-SYS程序对其条带模式进行分析,并构建树状图。在85%的相似度下,将分离菌株分为10个ARDRA类群。大多数分离株属于一组,其余分离株构成单独的组。不同作物根际的PSB随机分布。生化组与ARDRA组无相关性。
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