Conjugation Reaction between Citronellal and L-Tyrosine and Its Antimicrobial Properties against Bacteria and Fungi

Abstract

: The study of citronellal with L-tyrosin conjugation for antimicrobial properties has been conducted. The aim of this study to determine the relationship stucture between two compounds citronellal and L-tyrosine on antimicrobial activity against Staphylococcus aureus , Escherichia coli , and Candida albicans . The conjugation product obtained was yellow-white solid amorphous with the Rf value was 0.84 and the percentage of yield was 71.12%. The FT-IR spectra peak at 3205.69 cm -1 is represented the N-H stretching vibration from L-tyrosine, while the spectra appears at 1460.11 - 1438.90 cm -1 are represented the C=N which derived from imine or immonium from shift base reaction between citronellal and L-tyrosine. The GC-MS analysis showed that the peak 15 observed at RT 10.27 min. be expected a conjugation product with the m/z 316 [ M +H] + ion. The antimicrobial activity were determined by well diffusion method and the results showed that product of conjugation were have no antimicrobail activities at concentration tested.