Chemical composition and in vitro investigation of biological activities of Hemizygia bracteosa (Benth.) Briq leaves

M. Zinsou, Fidèle Assogba, F. Gbaguidi, M. Moudachirou
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引用次数: 4

Abstract

Hemizygia bracteosa (Benth.) Briq (Lamiaceae) is used in the treatment of mental disease, memory loss, HIV, malaria and had antimicrobial and hypoglycemic properties. To the authors’ knowledge, no scientific study has been done on these properties except antimicrobial and hypoglycemic properties. The phytochemical investigation focused on the quantification of phenolic compounds, flavonoids, tannins, anthocyanins and reducing sugars. Gas chromatography-mass spectrometry (GC-MS) analysis was performed on derivatized and non-derivatized extracts for the determination of chemical composition. Phytochemical composition evaluation  shows phenolics content between 9.3 and 24.8 mg eq GA/g, a flavonoids content of 9 to 10.5 mg eq Q/g, an anthocyanins content of 0.0005 to 0.0025 mg eq C3G/g, a reducing sugar content of 126.1 to 123.7 mg eq G/kg, and undetected tannin content. GC-MS results revealed the presence of sugars, alcohols, acids, sesquiterpenes, amino acids, ester, lactone, phenolic. It also revealed that acetylcholinesterase inhibition was between 16.9 and 36.5%; xanthine oxidase inhibition between 3.8 and 60.2% ; ii-amylase inhibition between 6.1 and 8.7%, anti-inflammatory activity between 4.7 and 8.1%, anti-cancer activity between 24 and 96.4% for MCF7 ; 33.1 and 96.8% for HCT116 ; antioxidant activity between 2.1 and 13.2%. The results of acetylcholinesterase inhibition of ethyl acetate and methanolic extracts may justify the use of the plant in the treatment of mental illnesses and memory loss. But H. bracteosa should be used with caution because of its very high anti-cancer activity that could induce cytotoxicity.   Key words: Hemizygia bracteosa, chemical composition, antioxidant, acetylcholinesterase, inflammation, xanthine oxidase, ii-amylase, cancer.
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小苞半镰刀菌的化学成分及体外生物活性研究Briq叶子
半苞叶(下)Briq (Lamiaceae)用于治疗精神疾病、记忆丧失、艾滋病毒、疟疾,并具有抗菌和降血糖的特性。据作者所知,除了抗菌和降血糖外,还没有对这些特性进行过科学研究。植物化学研究主要集中在酚类化合物、类黄酮、单宁、花青素和还原糖的定量分析。采用气相色谱-质谱(GC-MS)分析衍生化和非衍生化提取物的化学成分。植物化学成分评价表明,黄酮类化合物含量在9.3 ~ 24.8 mg eq GA/g之间,黄酮类化合物含量在9 ~ 10.5 mg eq Q/g之间,花青素含量在0.0005 ~ 0.0025 mg eq C3G/g之间,还原糖含量在126.1 ~ 123.7 mg eq g /kg之间,未检出单宁含量。GC-MS结果显示糖、醇、酸、倍半萜、氨基酸、酯、内酯、酚类物质的存在。乙酰胆碱酯酶抑制率为16.9% ~ 36.5%;黄嘌呤氧化酶抑制率为3.8% ~ 60.2%;MCF7的i-淀粉酶抑制率在6.1 ~ 8.7%之间,抗炎活性在4.7 ~ 8.1%之间,抗癌活性在24% ~ 96.4%之间;HCT116分别为33.1%和96.8%;抗氧化活性在2.1 - 13.2%之间。乙酰胆碱酯酶抑制乙酸乙酯和甲醇提取物的结果可能证明使用该植物治疗精神疾病和记忆丧失是合理的。但由于其具有很高的抗癌活性,可能引起细胞毒性,因此应谨慎使用。关键词:半苞草,化学成分,抗氧化剂,乙酰胆碱酯酶,炎症,黄嘌呤氧化酶,i-淀粉酶,癌症
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