Tissue culture optimization for Lallementia royleana L. an important medicinal plant

IF 0.4 4区生物学 Q4 Agricultural and Biological Sciences Iheringia Serie Botanica Pub Date : 2021-05-25 DOI:10.21826/2446-82312021V76E2021009

T. Mahmood, H. Arshad, W. Akhtar, M. Ilyas, Tariq Habib, Z. Shinwari

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Abstract

This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.

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重要药用植物扁豆的组织培养优化

本研究旨在建立一种高效的罗氏小扁豆离体培养方案，并分析各种激素对其培养能力的影响。评价了间接器官发生及其胼胝质形成能力;以1 mg L-1的苄基氨基嘌呤(BAP)和2 mg L-1的吲哚乙酸(IAA)组合为最佳(100%)。在直接再生中，叶片叶柄、节和节间外植体在不同浓度的BAP和动素(KIN)处理下进行拔节培养。当BAP 1.0 mg L-1, KIN 0.5 mg L-1时，茎节部位的发芽率最高(80%)。同样，1 mg L-1萘乙酸(NAA)的离体生根效果最好(24根)。小扁豆直接再生比胼胝质形成具有更高的再生能力。愈伤组织诱导、植株部位和激素组合之间存在显著差异(p = 0.002625)。2,4-二氯苯氧乙酸(2,4- d)与NAA、BAP与IAA组合处理后，茎节和节间外植体均表现出明显的愈伤组织反应。

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来源期刊

Iheringia Serie Botanica PLANT SCIENCES-

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Iheringia, Série Botânica is a publication edited by the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, since 1958. The name Iheringia is actually a tribute to Hermann von Ihering (a German researcher who became a Brazilian citizen) and his important studies in the Natural Sciences.The journal has the mission of publishing articles, reviews and original scientific notes that cover diverse areas in Botany, and may be written in Portuguese, Spanish and English. The journal is widely available in both international and national scientific community. The issues are published and distributed twice a year to 118 national institutions and 152 international institutions. The distribution is either free of charge or by exchange. Impac Factor JCR 2014/2015 = 0.263 Qualis CAPES 2015: B 4 (Biodiversity) B1 (Agricultural Science I) B1 (Environment)