FTIR Microspectroscopy in Agriculture

L. Pietrzak, S. Miller
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Abstract

Recently, the use and capabilities of NMR, UV-VIS and FTIR microspectroscopy in biology has increased. We apply UV-VIS μspectroscopy to localize isoflavones, β-glucans, proteins in cereal grains Using synchrotron FTIR microspectroscopy, we have mapped in situ some of the major chemical components (proteins, lipids and carbohydrates) in different tissues of cereal grains and mature soybean seeds The protein secondarystructure was also determined. NMR microimaging was applied to study water movement.
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农业中的FTIR显微光谱学
近年来,核磁共振、紫外-可见和红外光谱在生物领域的应用和能力不断提高。应用紫外可见光谱技术对谷物中的异黄酮、β-葡聚糖、蛋白质进行了定位。利用同步红外光谱技术,对谷物和成熟大豆种子中不同组织的主要化学成分(蛋白质、脂质和碳水化合物)进行了原位定位,并测定了蛋白质的二级结构。采用核磁共振微成像技术研究水的运动。
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