The Nucleotide Sequence of the tRNA{ie} from the Archaebacterium Thermoplasma acidophilum

Michael W. Kilpatrick, Richard T. Walker
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引用次数: 36

Abstract

The nucleotide sequence of Thermoplasma acidophilum tRNA{ie} has been shown to be: pGCCGGGGs4UGGCUCANCUGGAGGAGCm22GCCGGACm UCAUt6AAUCCGGAG-GUCUCGGGψψCmGAUCCCCGAUCCCGGCACCAoH. The tRNA contains eight modified nucleosides including the sequence ψψCm (54–56) which has been proposed as a unique feature of archaebacterial tRNAs. One modification is unknown but it is likely to be typically archaebacterial (N-15). Of the remaining four, one (s4U-8) is a typical eubac-terial modification and one (m22G-26) is a typical eukaryotic modification. The sequence shows little homology with any other published tRNA but shows almost 90% homology with the ancestral quasi-species sequence deduced by Eigen and Winter-Oswatitsch (1981).

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嗜酸热原菌tRNA{ie}的核苷酸序列
热原嗜酸菌tRNA{ie}的核苷酸序列为:pgccggs4uggcucancuggaggagcm22gccggacm ucaut6aauccggag - gucucgggψ cmgauccccgaucccggcaccaoh。tRNA包含8个修饰的核苷,其中包括序列ψ cm(54-56),这被认为是古细菌tRNA的独特特征。一种修饰是未知的,但很可能是典型的古细菌(N-15)。在剩下的四种中,一种(s4U-8)是典型的真核修饰,一种(m22G-26)是典型的真核修饰。该序列与其他已发表的tRNA几乎没有同源性,但与Eigen和Winter-Oswatitsch(1981)推断的祖先准物种序列几乎有90%的同源性。
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