Precision protein assays on compartmentalized biosensor arrays

Abstract

A biological measurement microsystem enables precision protein assays by compartmentalization of biosensors in a sensor array. Using a PDMS microfluidic interface on an 8 by 8 Giant Magnetoresistive sensor array, this compartmentalization technique performs all required measurements, including biological references, on a single sensor chip alongside the actual sample(s) to be measured. All data is acquired simultaneously on a single chip, circumventing a range of possible errors currently present in sequential biosensor measurement approaches. With our approach, we achieve a low concentration estimation error of 11%. Additionally this compartmentalization technique enables high throughput measurements using multiple samples on a single chip with a large-scale array of solid-state sensors.