Jingya Liu, Yinqiu Zheng, Shuang Dai, Li Li, Wei Wu, R. Gou, Deyuan Wang, Shi Long, Meihua Huang, Z. Xu
{"title":"In vitro Activity of Picroside I in Type 2 Diabetes Based on Oxidative Stress","authors":"Jingya Liu, Yinqiu Zheng, Shuang Dai, Li Li, Wei Wu, R. Gou, Deyuan Wang, Shi Long, Meihua Huang, Z. Xu","doi":"10.29356/jmcs.v67i2.1899","DOIUrl":null,"url":null,"abstract":"Abstract. The primary factor leading to insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is oxidative stress. Despite its liver-protecting, enzyme-lowering, immune-regulating, and antiviral effects, the impact of picroside I on oxidative stress, glucose utilization, and IR has not been investigated yet. In vitro studies were conducted to evaluate the antioxidant properties of different concentrations of picroside I. The results showed that picroside I effectively suppresses α-glucosidase and α-amylase with IC50 values of 109.75 μg/mL and 160.71 μg/mL in the range of 50-500 μg/mL. Additionally, when IR-HepG2 cells were treated with 80 μg/mL of picroside I, it was found to have little effect on cell viability, increase glucose consumption, decrease the levels of the free radical metabolite malonic dialdehyde, and increase superoxide dismutase activity. These findings indicate that picroside I has the potential to regulate oxidative stress in IR-HepG2 cells, potentially improving IR and exhibiting anti-T2DM activity.\nResumen. El factor principal que conduce a la resistencia a la insulina (IR) y a la diabetes mellitus tipo 2 (T2DM) es el estrés oxidativo. A pesar de sus efectos protectores del hígado, reductores de enzimas, inmunorreguladores y antivirales, aún no se ha investigado el impacto del picrósido I sobre el estrés oxidativo, la utilización de glucosa y la IR. Se realizaron estudios in vitro para evaluar las propiedades antioxidantes de diferentes concentraciones de picrósido I. Los resultados mostraron que el picrósido I suprime eficazmente la α-glucosidasa y la α-amilasa con valores IC50 de 109,75 μg/mL y 160,71 μg/mL en el rango de 50 -500 microgramos/ml. Además, cuando las células IR-HepG2 se trataron con 80 μg/mL de picrósido I, se encontró que tenía poco efecto sobre la viabilidad celular, aumentaba el consumo de glucosa, disminuía los niveles del metabolito de radicales libres dialdehído malónico y aumentaba la actividad de la superóxido dismutasa. Estos hallazgos indican que el picrósido I tiene el potencial de regular el estrés oxidativo en las células IR-HepG2, mejorando potencialmente la IR y exhibiendo actividad anti-T2DM.\n ","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":"24 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Mexican Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.29356/jmcs.v67i2.1899","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract. The primary factor leading to insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is oxidative stress. Despite its liver-protecting, enzyme-lowering, immune-regulating, and antiviral effects, the impact of picroside I on oxidative stress, glucose utilization, and IR has not been investigated yet. In vitro studies were conducted to evaluate the antioxidant properties of different concentrations of picroside I. The results showed that picroside I effectively suppresses α-glucosidase and α-amylase with IC50 values of 109.75 μg/mL and 160.71 μg/mL in the range of 50-500 μg/mL. Additionally, when IR-HepG2 cells were treated with 80 μg/mL of picroside I, it was found to have little effect on cell viability, increase glucose consumption, decrease the levels of the free radical metabolite malonic dialdehyde, and increase superoxide dismutase activity. These findings indicate that picroside I has the potential to regulate oxidative stress in IR-HepG2 cells, potentially improving IR and exhibiting anti-T2DM activity.
Resumen. El factor principal que conduce a la resistencia a la insulina (IR) y a la diabetes mellitus tipo 2 (T2DM) es el estrés oxidativo. A pesar de sus efectos protectores del hígado, reductores de enzimas, inmunorreguladores y antivirales, aún no se ha investigado el impacto del picrósido I sobre el estrés oxidativo, la utilización de glucosa y la IR. Se realizaron estudios in vitro para evaluar las propiedades antioxidantes de diferentes concentraciones de picrósido I. Los resultados mostraron que el picrósido I suprime eficazmente la α-glucosidasa y la α-amilasa con valores IC50 de 109,75 μg/mL y 160,71 μg/mL en el rango de 50 -500 microgramos/ml. Además, cuando las células IR-HepG2 se trataron con 80 μg/mL de picrósido I, se encontró que tenía poco efecto sobre la viabilidad celular, aumentaba el consumo de glucosa, disminuía los niveles del metabolito de radicales libres dialdehído malónico y aumentaba la actividad de la superóxido dismutasa. Estos hallazgos indican que el picrósido I tiene el potencial de regular el estrés oxidativo en las células IR-HepG2, mejorando potencialmente la IR y exhibiendo actividad anti-T2DM.
摘要导致胰岛素抵抗(IR)和2型糖尿病(T2DM)的主要因素是氧化应激。尽管其具有保肝、降酶、免疫调节和抗病毒作用，但其对氧化应激、葡萄糖利用和IR的影响尚未被研究。结果表明，在50 ~ 500 μg/mL范围内，picro甙I能有效抑制α-葡萄糖苷酶和α-淀粉酶，IC50值分别为109.75和160.71 μg/mL。此外，80 μg/mL的picroside I处理IR-HepG2细胞时，发现对细胞活力影响不大，增加葡萄糖消耗，降低自由基代谢产物丙二醛水平，增加超氧化物歧化酶活性。这些发现表明，picroside I具有调节IR- hepg2细胞氧化应激的潜力，可能改善IR并表现出抗2型糖尿病的活性。El因子对胰岛素抵抗(IR)、2型糖尿病(T2DM)和应激氧化的影响。一项研究表明，通过抗病毒药物可以保护del hígado，还原enzimas，免疫调节，aún，目前还没有研究对del picrósido的影响，因为它可以抑制氧化，通过IR可以降低葡萄糖含量utilización。研究了不同浓度丙烯类抗氧化剂的体外对乙酰氨基葡萄糖苷(picrósido 1 . Los resultados mostraronque el picrósido 1) α-葡萄糖苷(α-amilasa con valores) IC50 de 109、75 μg/mL、160、71 μg/mL和50 ~ 500 μg/mL的乙酰氨基葡萄糖苷的体外对乙酰氨基葡萄糖苷(α-amilasa con valores)的体外对活性的影响。Además, cuando las csamulas IR-HepG2 se trataron con 80 μg/mL de picrósido I, se encontró que tenía poco effecto sobre la viabilidad cell, aumentaba el consumo de glucosa, disminuía los niveles del metabolito de radicales libres dialdehído malónico y aumentaba la actividad de la superóxido dismutasa。Estos hallazgos indican que el picrósido研究了正常应激细胞的氧化电位与正常应激细胞IR- hepg2的氧化电位、正常应激细胞IR- hepg2的氧化电位、正常应激细胞IR的活性及抗t2dm的作用。
期刊介绍：
The Journal of the Mexican Chemical Society (J. Mex. Chem. Soc.) is a scientific, blind, peer reviewed, and open access, free of charge publication that covers all areas of chemistry and its sub-disciplines (i.e. medicinal chemistry, natural products, electrochemistry, material science, computational chemistry, organic chemistry, bionirganic chemistry, etc). It is devoted to facilitating the worldwide advancement of our understanding of chemistry. It will primarily publish original contributions of research in all branches of the theory and practice of chemistry in its broadest context as well as critical reviews in active areas of chemical research where the author has published significant contribution. The J. Mex. Chem. Soc. is a quarterly publication which language of submission and publication is English. To be suitable for publication in J. Mex. Chem. Soc., manuscripts must describe novel aspects of chemistry, high quality of results and discussion an excellent bibliographic support, and contribute to the development of the field. Routine or incremental work are not suitable for publication in J. Mex. Chem. Soc. Authors are encouraged to send contributions in electronic form. Our online submission system guides you stepwise through the process of entering your article details and uploading your files.
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