Genetic diversity and gene-pool of Medicago polymorpha L. based on retrotransposon-based markers

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences Caryologia Pub Date : 2022-07-07 DOI:10.36253/caryologia-1428

H. Jing, S. Esfandani-Bozchaloyi

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Abstract

The genus Medicago L. (Fabaceae) comprises approximately 87 different species of herbs and shrubs widespread from the Mediterranean to central Asia. Medicago polymorpha is a herbaceous legume that can be a useful pasture plant, in particular, in regions with a Mediterranean climate. It had aroused great interest due to high nutritious quality, highly palatability and N-fixing plan in neutral soil. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Due to the medicinal importance of this species, a genetic variability and populations’ structure study is performed studying 15 geographical populations of Medicago polymorpha. Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this plant. AMOVA test produced significant genetic difference (PhiPT = 0.46, P = 0.010) among the studied populations and also revealed that, 66% of total genetic variability was due to within population diversity while, 34% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. STRUCTURE analyses and population assignment test revealed some degree of gene flow among these populations. PCoA plot of populations was in agreement with UPGMA clustering of molecular data. These results indicated that geographical populations of Medicago polymorpha are well differentiated based on (IRAP) markers.

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基于反转录转座子标记的紫花苜蓿遗传多样性及基因库研究

紫花苜蓿属(豆科)由大约87种不同的草本植物和灌木组成，分布于地中海到中亚。多形紫花苜蓿是一种草本豆科植物，可以作为一种有用的牧场植物，特别是在地中海气候地区。由于其高营养品质、高适口性和在中性土壤中的固氮能力，引起了人们的极大兴趣。在伊朗没有关于其种群遗传结构、遗传多样性和形态变异的信息。由于该物种具有重要的药用价值，本文对紫花苜蓿15个地理居群进行了遗传变异和居群结构研究。因此，我们利用6个反转录转座子间扩增多态性(IRAP)标记和15个IRAP组合标记来揭示该植物群体内和群体间的遗传多样性。AMOVA检验在研究群体间产生了显著的遗传差异(PhiPT = 0.46, P = 0.010)，也表明66%的遗传变异是由群体内多样性引起的，34%的遗传变异是由群体间的遗传分化引起的。Mantel检验表明，遗传距离与地理距离呈正相关。结构分析和群体分配检验表明，这些群体之间存在一定程度的基因流动。群体的PCoA图与分子数据的UPGMA聚类一致。这些结果表明，利用IRAP标记可以很好地分化多花苜蓿的地理居群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.