Cannabidiol modulates contextual fear memory consolidation in animals with experimentally induced type-1 diabetes mellitus.

IF 3.8 4区 医学 Q1 Medicine Acta Neuropsychiatrica Pub Date : 2024-10-01 Epub Date: 2023-02-20 DOI:10.1017/neu.2023.13
Yane Costa Chaves, Ana Maria Raymundi, Ana Paula Farias Waltrick, José Alexandre de Souza Crippa, Cristina Aparecida Jark Stern, Joice Maria da Cunha, Janaína Menezes Zanoveli
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Abstract

Objectives: In view of the neuroprotective characteristic of cannabidiol (CBD) and its beneficial action on aversive memory in non-diabetic animals, we aimed to investigate in animals with experimentally induced type-1 diabetes mellitus (T1DM) whether CBD treatment would be able to impair the contextual fear memory consolidation, its generalisation and whether the effect would be lasting. We also investigated the CBD effect on anxiety-like responses.

Methods: After T1DM induction, animals received single or more prolonged treatment with CBD and were submitted to the contextual fear conditioning test. As expression of activity-regulated cytoskeletal-associated (Arc) protein is necessary for memory consolidation, we evaluated its expression in the dorsal hippocampus (DH). For evaluating anxiety-related responses, animals were submitted to the elevated plus maze test (EPMT), in which the time and number of entries in the open arms were used as anxiety index.

Results: A single injection of CBD impaired the contextual fear memory consolidation and its generalisation, which was evaluated by exposing the animal in a neutral context. This single injection was able to reduce the elevated expression of Arc in the DH from these animals. Interestingly, more prolonged treatment with CBD also impaired the persistence of context-conditioned fear memory and induced an anxiolytic-like effect, as the treated group spent more time in the open arms of the EPMT.

Conclusion: CBD interferes with contextual fear memory and the dosage regimen of treatment seems to be important. Moreover, we cannot rule out the involvement of emotional aspects in these processes related to fear memory.

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大麻二酚可调节实验诱导的1型糖尿病动物的情境恐惧记忆巩固。
研究目的鉴于大麻二酚(CBD)的神经保护特性及其对非糖尿病动物的厌恶记忆的有益作用,我们旨在研究在实验诱导的 1 型糖尿病(T1DM)动物中,CBD 治疗是否能够损害情境恐惧记忆的巩固、泛化以及这种影响是否持久。我们还研究了 CBD 对焦虑样反应的影响:方法:T1DM诱导后,动物接受单次或多次CBD治疗,并接受情境恐惧条件反射测试。由于活性调节细胞骨架相关蛋白(Arc)的表达是记忆巩固的必要条件,我们评估了其在海马背侧(DH)的表达。为了评估与焦虑相关的反应,我们对动物进行了高架加迷宫试验(EPMT),其中进入开放臂的时间和次数被用作焦虑指数:结果:单次注射 CBD 会损害情境恐惧记忆的巩固及其泛化,评估方法是将动物置于中性情境中。单次注射能够降低这些动物 DH 中 Arc 表达的升高。有趣的是,更长时间的CBD治疗也会损害情境条件恐惧记忆的持续性,并诱发类似抗焦虑的效果,因为治疗组在EPMT张开的怀抱中度过的时间更长:结论:CBD会干扰情境恐惧记忆,治疗剂量似乎很重要。此外,我们还不能排除这些与恐惧记忆有关的过程中存在情绪因素。
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来源期刊
Acta Neuropsychiatrica
Acta Neuropsychiatrica 医学-精神病学
CiteScore
8.50
自引率
5.30%
发文量
30
审稿时长
6-12 weeks
期刊介绍: Acta Neuropsychiatrica is an international journal focussing on translational neuropsychiatry. It publishes high-quality original research papers and reviews. The Journal''s scope specifically highlights the pathway from discovery to clinical applications, healthcare and global health that can be viewed broadly as the spectrum of work that marks the pathway from discovery to global health.
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