Melanogenesis inhibitory effect of Perilla Frutescens (L.) Britt on a hyperpigmentation model in rabbit

Q4 Pharmacology, Toxicology and Pharmaceutics Pharmaceutical Sciences Asia Pub Date : 2022-01-01 DOI:10.29090/psa.2022.03.22.010

Ngoc Phuc Nguyen, Thanh-Hao Do, Le-Y Nguyen, Thi Ly Thu Pham, N. Huynh

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Abstract

The study aims to evaluate the melanogenesis inhibitory action of the ethyl acetate (EA) extract from Perilla frutescens (L.) Britt on a hyperpigmented rabbit model induced by UVA-exposure combined to progesterone injection. The dorsal shaved skin of the experimental rabbits was exposed to UVA radiation (386 nm, 147 uW/cm 2 ) in 30 minutes each day for 28 days. Progesterone was intramuscularly injected every other day at the dose of 5 mg/kg. EA extract was topically applied daily from the first day of melasma appearance until the end of the experiment. Morphometric observation based on color thresholder application on MATLAB software. Moreover, melanin determination and histological analysis were also performed. The morphometric observation on the surface of melasma areas as well as the histological analysis from 5% EA-treated group are similar to these of 4% Hydroquinone-treated group at day 14, 21, 28. Melanin concentration in 5% EA-treated group was significantly decreased as compared to the hyperpigmentation group. In conclusion, EA extract of Perilla leaves clearly exhibited the inhibitory effect on melanogenesis in the hyperpigmented rabbit-induced by UVA exposure combined to progesterone injection.

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紫苏对黑素生成的抑制作用兔色素沉着过度模型的建立

本研究旨在评价紫苏乙酸乙酯(EA)提取物的抑黑作用。uva暴露联合黄体酮注射致兔色素沉着模型的研究。实验兔背部剃光皮肤，每天30分钟暴露于UVA辐射(386 nm, 147 uW/ cm2)，连续28天。每隔一天肌肉注射黄体酮，剂量为5mg /kg。从黄褐斑出现的第一天起，每日局部涂抹EA提取物，直至实验结束。基于颜色阈值的形态测量观察在MATLAB软件中的应用。此外，还进行了黑色素测定和组织学分析。5% ea组与4%对苯二酚组在第14、21、28天的黄褐斑表面形态学观察和组织学分析相似。5% ea治疗组黑色素浓度较色素沉着组明显降低。综上所述，紫苏叶EA提取物对UVA联合黄体酮诱导的高色素家兔黑色素生成有明显的抑制作用。

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来源期刊

Pharmaceutical Sciences Asia Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Pharmaceutical Sciences Asia (PSA) journal is a double-blinded peer-reviewed journal in English published quarterly, by the Faculty of Pharmacy, Mahidol University, Thailand. The PSA journal is formerly known as Mahidol University Journal of Pharmaceutical Sciences and committed to the timely publication of innovative articles and reviews. This journal is available in both printed and electronic formats. The PSA journal aims at establishing a publishing house that is open to all. It aims to disseminate knowledge; provide a learned reference in the field; and establish channels of communication between academic and research expert, policy makers and executives in industry and investment institutions. The journal publishes research articles, review articles, and scientific commentaries on all aspects of the pharmaceutical sciences and multidisciplinary field in health professions and medicine. More specifically, the journal publishes research on all areas of pharmaceutical sciences and related disciplines: Clinical Pharmacy Drug Synthesis and Discovery Targeted-Drug Delivery Pharmaceutics Biopharmaceutical Sciences Phytopharmaceutical Sciences Pharmacology and Toxicology Pharmaceutical Chemistry Nutraceuticals and Functional Foods Natural Products Social, Economic, and Administrative Pharmacy Clinical Drug Evaluation and Drug Policy Making Antimicrobials, Resistance and Infection Control Pharmacokinetics and Pharmacodynamics.