Detection of MTHFR (A1298C) Gene Polymorphism in Women Suffer from Different Types of Abortion in Mosul City

Abstract

Genetic basis of recurrent abortion is poorly understood, Single gene mutations, polygenic, and cytogenetic factors are all found to show association with recurrent abortion. The analysis of single nucleotide polymorphisms in the genes coding for enzymes which responsible for regulatory of metabolic pathways (e.g. methylene tetra hydrofolate reductase), clotting factors (Factor V leiden and prothrombin) and hormones and hormone receptors (progesterone receptor). This study included (85) pregnant women who suffer from problems during pregnancy and the age range was between (25-35) years. The samples were divided into four groups ,the first included pregnant women who suffer from recurrent spontaneous abortion , while the second included pregnant women with missed abortion , the third group included pregnant women with recurrent spontaneous abortion and positive TORCH examination and the final group was considered a control group. The result of the study showed a relationship between cases of pregnancy disorder and genetic mutations of MTHFR gene at the (A1298C) site, the result of the study for a group of women with RSA showed that the distribution of the mutant genotype CC was the highest 40% and the repetitive alleles for this group was 57.5% for mutant allele C and 42.5% for normal allele A. Also the group of missed abortion showed that the distribution of the mutant genotype CC was the highest 40% and the repetitive alleles for this group was 52.5% for mutant allele C and 47.5% for normal allele A. Whereas the group of women with positive TORCH showed that the distribution of heterozygous genotype was the highest 50% and the repetitive alleles for this group was 32.2% for mutant allele C and 67.8% for normal allele A.