Development of Disease-resistant Japonica Rice Varieties and Effects of Pyramiding Resistance Genes

Woo-Jae Kim, Man-Kee Baek, Hyeong-Su Park, Geon-Mi Lee, Chang-Min Lee, Seok-Man Kim, Young-chan Cho, Jeonghwan Seo, O. Jeong
{"title":"Development of Disease-resistant Japonica Rice Varieties and Effects of Pyramiding Resistance Genes","authors":"Woo-Jae Kim, Man-Kee Baek, Hyeong-Su Park, Geon-Mi Lee, Chang-Min Lee, Seok-Man Kim, Young-chan Cho, Jeonghwan Seo, O. Jeong","doi":"10.7740/KJCS.2020.65.4.314","DOIUrl":null,"url":null,"abstract":"This study was carried out to develop a resistant variety against the K3a race of bacterial blight, Xanthomonas oryzae pv. oryzae, through expansion and pyramiding of resistance genes. To develop an elite bacterial blight-resistant cultivar, the breeding process and bacterial blight resistance reactions in advanced backcross lines (ABLs) were analyzed. ABLs21 which contain Xa3 and Xa21, were developed by double backcrossing japonica cultivar Hwanggeumnuri, which has bacterial blight resistant Xa3 gene, and indica variety IRBB21, which havs Xa21 gene, followed by disease resistance bioassay and marker-assisted selection. The resistance genes of ABLs21 were amplified by PCR with the molecular markers 9643.T4 (Xa3) and U1/I1 (Xa21). Hwanggeumnuri and IRBB3 showed resistance reactions against K1, K2, and K3 races, and a susceptible reaction against K3a, K4, and K5 races. IRBB21 showed resistance reactions against K2, K3, K3a, K4 and K5 races, and a susceptible reaction against K1 race. Hwanggeumnuri showed susceptible reactions at the seedling, tillering and adult stages (all stages), whereas ABL21-1 showed moderate resistance at the tillering stage. ABL21-1 showed stable resistance against 18 isolates of K3a race, and the lesion length was shorter than that of the donor parents. In cluster analysis, the HB4032 isolate showed the highest pathogenicity among the 18 isolates. The molecular marker polymorphisms and average substituted chromosome segment lengths of ABLs21 were 63.2 % and 86.1 cM, respectively. Insertion of the donor chromosomal segments occurred in the predicted region of the Xa21 gene of ABLs21.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"16 1","pages":"314-326"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Korean Journal of Crop Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7740/KJCS.2020.65.4.314","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

This study was carried out to develop a resistant variety against the K3a race of bacterial blight, Xanthomonas oryzae pv. oryzae, through expansion and pyramiding of resistance genes. To develop an elite bacterial blight-resistant cultivar, the breeding process and bacterial blight resistance reactions in advanced backcross lines (ABLs) were analyzed. ABLs21 which contain Xa3 and Xa21, were developed by double backcrossing japonica cultivar Hwanggeumnuri, which has bacterial blight resistant Xa3 gene, and indica variety IRBB21, which havs Xa21 gene, followed by disease resistance bioassay and marker-assisted selection. The resistance genes of ABLs21 were amplified by PCR with the molecular markers 9643.T4 (Xa3) and U1/I1 (Xa21). Hwanggeumnuri and IRBB3 showed resistance reactions against K1, K2, and K3 races, and a susceptible reaction against K3a, K4, and K5 races. IRBB21 showed resistance reactions against K2, K3, K3a, K4 and K5 races, and a susceptible reaction against K1 race. Hwanggeumnuri showed susceptible reactions at the seedling, tillering and adult stages (all stages), whereas ABL21-1 showed moderate resistance at the tillering stage. ABL21-1 showed stable resistance against 18 isolates of K3a race, and the lesion length was shorter than that of the donor parents. In cluster analysis, the HB4032 isolate showed the highest pathogenicity among the 18 isolates. The molecular marker polymorphisms and average substituted chromosome segment lengths of ABLs21 were 63.2 % and 86.1 cM, respectively. Insertion of the donor chromosomal segments occurred in the predicted region of the Xa21 gene of ABLs21.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
粳稻抗病品种的选育及抗锥体基因的作用
本研究旨在培育一种抗水稻白叶枯病菌(Xanthomonas oryzae pv) K3a小种的抗性品种。通过抗性基因的扩增和锥体化。为培育优良的白叶枯病抗性品种,对先进回交系的选育过程和抗白叶枯病反应进行了分析。以具有抗白叶枯病基因Xa3的粳稻品种黄金花和具有Xa21基因的籼稻品种IRBB21为材料,经抗病生物测定和标记辅助选择,获得了含有Xa3和Xa21的ABLs21。用PCR扩增ABLs21的抗性基因,标记为9643。T4 (Xa3)和U1/I1 (Xa21)。黄锦葵和IRBB3对K1、K2和K3小种呈抗性反应,对K3a、K4和K5小种呈敏感反应。IRBB21对K2、K3、K3a、K4和K5小种表现出抗性反应,对K1小种表现出敏感反应。黄锦菊在苗期、分蘖期和成虫期均表现出敏感反应,而ABL21-1在分蘖期表现为中等抗性。ABL21-1对18株K3a小种表现出稳定的抗性,且病变长度比供体亲本短。聚类分析结果显示,HB4032分离株的致病性最高。ABLs21的分子标记多态性和平均取代染色体片段长度分别为63.2%和86.1 cM。供体染色体片段的插入发生在ABLs21的Xa21基因的预测区域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Comparative Transcriptome Analysis of the Response of Two Lines of Rapeseed (Brassica napus L.) to Cold Stress Evaluation on the Effects of Deicing Salts on Crop using Seedling Emergence Assay of Oilseed Rape (Brassica napus) Review on Adaptability of Rice Varieties and Cultivation Technology According to Climate Change in Korea Investigation of Changes in Grain Quality and Physicochemical Properties of Rice According to the Temperature during the Ripening Stage and Preharvest Sprouting Genetic Diversity and Population Structure of Peanut (Arachis hypogaea L.) Accessions from Five Different Origins
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1