Developing an electrochemical aptasensor using a specific aptamer to detect neomycin antibiotic in milk

Abstract

Milk and dairy products, often considered healthy and nutritionally balanced natural foods, include essential nutrients for all ages and are an important part of the daily diet. However, the indiscriminate use of antibiotics, more than the allowable dose, and improper purposes makes food and animal products in general and dairy at risk of harming human health. Sensitive consumers may experience allergies or even more severe health problems. Antibiotics are left over for a long time, and it will lead to antibiotic resistance, making it difficult for people to control diseases and causing many consequences in the future. There are many modern methods proposed to detect antibiotics in food. One of the main methods is the electrochemical method. In this study, we used aptamer Neo6 and gold nanochip to fabricate an electrochemical aptasensor capable of determining neomycin residues in milk with a linear range of 10 - 3,000 ng/mL as determining the accuracy of the neomycin residues in milk. The sensitivity and specificity of the aptasensor are quite high. In addition, we initially compared over 20 milk samples supplemented with antibiotics, showing similar results with those analyzed by LC-MS/MS method.