Antibacterial Effect of Turkish Black Cumin Seed Oil Against Pathogen Bacteria's

Ilyas Guldal, Ş. Karabıyıklı
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Abstract

Black cumin seeds are used for medicinal and aromatic purposes in Turkey. Black cumin seed oil (BSCO) was provided from a local producer. BCSO was extracted by cold-pressing method. Antibacterial effect of BCSO was investigated against B. cereus (ATCC 10876), L. monocytogenes (ATCC 19115), E. coli (ATCC 25922), Salmonella Thypimurium (ATCC 14028) and S. aureus (ATCC 25923) by minimum inhibitory concentration (MIC) method. MIC values were determined according to micro tube dilution method. BCSO had antibacterial effect against B. cereus and S. aureus and didn’t have antibacterial effect against other bacterias. B. cereus and S. aureus are Gram (+) and others are Gram (-). In various studies, it had been found that Gram (+) bacterias are less resistance to aromatic oils. Fatty acid composition of BCSO was investigated for additional information of material and major fatty acids of BCSO are linoleic acid, oleic acid and palmitic acid. As a results, study suggests that cold-pressed BCSO can be used for its functional property. Pract?cal Appl?cat?ons Black seed oil is widely used in ethnopharmacology. It is marketed as a treatment agent for many mild diseases in the shops called "Aktar" in Turkish culture and it finds a response by the public. However, its use in industry is limited due to its pungent odor and taste. In this study, the functional properties of black cumin oil were investigated.
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土耳其黑孜然籽油对病原菌的抑菌作用
黑孜然种子在土耳其用于药用和芳香目的。黑孜然籽油(BSCO)由当地生产商提供。采用冷压法提取BCSO。采用最小抑菌浓度法(MIC)研究BCSO对蜡样芽孢杆菌(ATCC 10876)、单核增生乳杆菌(ATCC 19115)、大肠杆菌(ATCC 25922)、水蛭沙门氏菌(ATCC 14028)和金黄色葡萄球菌(ATCC 25923)的抑菌效果。采用微管稀释法测定MIC值。BCSO对蜡样芽孢杆菌和金黄色葡萄球菌有抑菌作用,对其他细菌无抑菌作用。蜡样芽孢杆菌和金黄色葡萄球菌为革兰氏(+),其他为革兰氏(-)。在各种研究中,已经发现革兰氏(+)细菌对芳香油的抗性较低。对BCSO的脂肪酸组成进行了研究,发现BCSO的主要脂肪酸为亚油酸、油酸和棕榈酸。因此,研究表明,冷压BCSO具有良好的功能特性,可用于生产。Pract吗?卡尔:猫?黑籽油在民族药理学中有着广泛的应用。它在土耳其文化中被称为“Aktar”的商店里作为治疗许多轻微疾病的药物进行销售,并得到了公众的响应。然而,由于其刺鼻的气味和味道,它在工业上的使用受到限制。本研究对黑孜然油的功能特性进行了研究。
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