The expression and significance of long-chain non-coding RNA linc00261 in non-small cell lung cancer

Abstract

Objective To investigate the expression of long-chain non-coding RNA (lncRNA) linc00261 in non-small cell lung cancer (NSCLC) and its clinical significance. Methods Included in 100 patients with NSCLC were admitted to Haikou Hospital Affiliated to Xiangya School of Medicine, Central South University from February 2017 to September 2018 were enrolled in this study.Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA linc00261 in cancer tissues and adjacent tissues of patients, and to analyze the relationship between the expression of lncRNA linc00261 and clinicopathological characteristics of patients.The expression plasmid of lncRNA linc00261 was designed and synthesized and transfected into A549 cells.The expression of lncRNA linc00261 in transfected cells was detected by qRT-PCR, the proliferation of transfected cells was detected by MTT, and the apoptosis of transfected cells was detected by flow cytometry. Results The results of qRT-PCR showed that the average relative expression of lncRNA linc0026 1 in cancer tissues of 100 NSCLC patients was significantly lower than that in adjacent tissues (1.19±0.23), with statistical significance (t=7.753, P 0.05), but the relative expression of lncRNA linc00261 in cancer tissues of NSCLC patients with different tissue differentiation degree, clinical stage and lymph node metastasis had statistical significance (all P 0.05). After 48 hours, 72 hours and 96 hours of transfection, the cell proliferation of A549 cells transfected with the expression plasmid of lncRNA linc00261 was significantly lower than that of the negative control group and the blank control group (t=3.187, 5.549, both P<0.05). The results of flow cytometry showed that the apoptotic rate of A549 cells transfected with the expression plasmid of lncRNA linc00261 was significantly higher than that of the negative control group and the blank control group after 48 hours of transfection (χ2=4.175, 6.093, both P<0.05). Conclusions lncRNA linc00261 is down-regulated in cancer tissues of NSCLC patients and is related to the development of NSCLC.It may play an anti-oncogene role in NSCLC.Overexpression of lncRNA linc0026 can inhibit the proliferation and induce apoptosis of NSCLC cells, which may become a potential therapeutic target of NSCLC. Key words: Carcinoma, non-small-cell lung; Cell proliferation; Apoptosis; Long-chain non-coding RNA; linc00261