Structure and Functions of Chromosomal and Extrachromosomal DNA in Halobacteria

Felicitas Pfeifer, Klaus Ebert, Gottfried Weidinger, Werner Goebel
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引用次数: 15

Abstract

The genomic DNA of Halobacterium halobium and several other extremely halophilic species is separated on bisacrylamide/malachitegreen columns into two fractions which differ considerably in their GC content. It is shown that the AT richer fraction exhibits a high degree of variability and often forms circular DNA elements of various sizes. The major circular DNA behaves like a plasmid in that it is present in a constant number of copies. Insertions of IS-like elements of different lengths seem to be responsible for the genetic variability. These insertions are reversible in some instances. This has allowed us to localize the genetic determinant for gas vesicle formation on the major circular DNA.

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盐杆菌中染色体和染色体外DNA的结构和功能
在双丙烯酰胺/孔雀石绿色谱柱上,将Halobacterium halobium和其他几种极端嗜盐物种的基因组DNA分离成两个GC含量差异很大的组分。结果表明,富含AT的组分表现出高度的可变性,并经常形成各种大小的环状DNA元件。主要的环状DNA表现得像质粒,因为它以固定数量的拷贝存在。不同长度的is样元素的插入似乎是造成遗传变异的原因。在某些情况下,这些插入是可逆的。这使我们能够在主要的环状DNA上定位气泡形成的遗传决定因素。
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