Microbodies in the Brown Rot Fungus Poria contigua

Stephanie Bringer, Hans-Peter Bochem, Benno Sprey, Hermann Sahm
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引用次数: 2

Abstract

Using the diaminobenzidine (DAB) reaction catalase activity could be demonstrated cytochemically in cytoplasmic structures of Poria contigua bearing the general ultrastructural features of microbodies. These kidney-shaped compartments were associated with a densely packed coherent membrane system. When H2O2 was replaced by methanol the microbodies were stained indicating that alcohol oxidase generated the H2O2 for the peroxidative conversion of DAB by catalase. Based on these results we assume that catalase and alcohol oxidase are located in microbodies of this fungus as found for methanol-utilizing yeasts.

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褐腐菌中的微体
用二氨基联苯胺(DAB)反应可以在具有微体一般超微结构特征的茯苓细胞质结构中证实过氧化氢酶活性。这些肾状隔室与密集排列的相干膜系统相关。当用甲醇代替H2O2时,微体染色表明乙醇氧化酶产生H2O2,过氧化氢酶对DAB进行过氧化转化。根据这些结果,我们推测过氧化氢酶和酒精氧化酶与利用甲醇的酵母一样存在于真菌的微体中。
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