A rapid, simple and simultaneous detection of 2-keto-, 5-keto-and 2,5-diketogluconic acids by thin-layer chromatography in culture media of acetic acid bacteria
{"title":"A rapid, simple and simultaneous detection of 2-keto-, 5-keto-and 2,5-diketogluconic acids by thin-layer chromatography in culture media of acetic acid bacteria","authors":"F. Gosselé, J. Swings, J. De Ley","doi":"10.1016/S0172-5564(80)80039-X","DOIUrl":null,"url":null,"abstract":"<div><p>A rapid and simple test for the simultaneous detection of 2-keto-, 5-keto- and 2.5-diketo-gluconic acids by thin-layer chromatography and their revelation with o-phenylene-diamine. HCl is described. 32 <em>Acetobacter</em> and 83 <em>Gluconobacter</em> strains were scanned with this method. The formation of keto-gluconic acids appears to be a stable and reliable feature for the classification of acetic accid bacteria.</p></div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 2","pages":"Pages 178-181"},"PeriodicalIF":0.0000,"publicationDate":"1980-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80039-X","citationCount":"57","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S017255648080039X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 57
Abstract
A rapid and simple test for the simultaneous detection of 2-keto-, 5-keto- and 2.5-diketo-gluconic acids by thin-layer chromatography and their revelation with o-phenylene-diamine. HCl is described. 32 Acetobacter and 83 Gluconobacter strains were scanned with this method. The formation of keto-gluconic acids appears to be a stable and reliable feature for the classification of acetic accid bacteria.