A rapid, simple and simultaneous detection of 2-keto-, 5-keto-and 2,5-diketogluconic acids by thin-layer chromatography in culture media of acetic acid bacteria

F. Gosselé, J. Swings, J. De Ley
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引用次数: 57

Abstract

A rapid and simple test for the simultaneous detection of 2-keto-, 5-keto- and 2.5-diketo-gluconic acids by thin-layer chromatography and their revelation with o-phenylene-diamine. HCl is described. 32 Acetobacter and 83 Gluconobacter strains were scanned with this method. The formation of keto-gluconic acids appears to be a stable and reliable feature for the classification of acetic accid bacteria.

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醋酸菌培养基中2-酮-、5-酮-和2,5-二酮葡萄糖酸的薄层色谱快速、简便、同时检测
用薄层色谱法同时检测2-酮-、5-酮-和2.5-二酮-葡萄糖酸及其邻苯二胺的揭示。描述了HCl。用该方法对32株醋酸杆菌和83株葡萄糖杆菌进行了扫描。酮-葡萄糖酸的形成似乎是醋酸细菌分类的一个稳定可靠的特征。
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