Untersuchungen zum Pentose- und Pentitolstoffwechsel bei Staphylococcus xylosus und Staphylococcus saprophyticus

A. Lehmer, K.H. Schleifer
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引用次数: 2

Abstract

The degradation of pentoses and xylitol, respectively is a meaningful taxonomic parameter for the distinction of the coagulase-negative staphylococci Staphylococcus xylosus and S. saprophyticus. It is, therefore, of interest to learn more about the catabolism of these C5-sugars and sugar alcohols. The following pathway for the breakdown of pentoses and xylitol has been found in S. xylosus and S. saprophyticus:

inducible uptake of unsubstituted carbohydrates; phosphoenolpyruvate phosphotransferase system (PTS) is not involved in the uptake of pentoses and xylitol.

Isomerization of pentoses and dehydration of xylitol is inducible. Phosphorylation probably occurs on ketopentoses, since activity for D-ribulokinase could be detected in D-ribose grown cells, but neither activity for pentokinases nor D-ribose-5-phosphate-isomerase could be demonstrated.

Therefore, the pathway is analogous to the metabolism of pentoses and pentitol in Enterobacteriaceae, exceptionally for D-ribose, and differs from the degradation of pentitols by Lactobacillaceae. Growth with D-xylose as substrate was inhibited by xylitol due to the inhibition of the enzyme D-xylose-isomerase.

Experiments with mutants should prove the value of pentose/pentitol utilization as a biochemical character for the identification of S. xylosus and S. saprophyticus. It was possible to isolate pentose-negative mutants of S. xylosus which resemble S. saprophyticus in the pentose/pentitol metabolism, but we did not succeed in isolating pentose-positive mutant of S. saprophyticus. This indicates that degradation of xylose and/or arabinose is a characteristic property fo S. xylosus strains. Pentose-negative mutants of S. xylosus lack activity for the isomerization of pentoses but they are still typical S. xylosus as could be shown by DNA-DNA-hybridization studies. DNA-homology values between wild and mutant strains were about 100%, whereas homology values between these mutant strains and a pentose-negative S. saprophyticus wild type were 35–40% corresponding to values obtained between wild types of S. xylosus and S. saprophyticus.

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葡萄球菌乳腺新陈代谢检查
戊糖和木糖醇的降解是区分凝固酶阴性葡萄球菌木糖葡萄球菌和腐生葡萄球菌的一个有意义的分类参数。因此,更多地了解这些c5糖和糖醇的分解代谢是很有意义的。木糖酵母和腐生酵母分解戊糖和木糖醇的途径如下:诱导吸收未取代的碳水化合物;磷酸烯醇丙酮酸磷酸转移酶系统(PTS)不参与戊糖和木糖醇的摄取。诱导戊糖异构化和木糖醇脱水。磷酸化可能发生在酮酮糖上,因为在d -核糖生长的细胞中可以检测到d -核糖体激酶的活性,但不能证明戊激酶和d -核糖体-5-磷酸异构酶的活性。因此,该途径类似于肠杆菌科戊糖和戊醇的代谢,但d -核糖例外,不同于乳酸杆菌科对戊糖醇的降解。木糖醇对d -木糖异构酶的抑制作用抑制了以d -木糖为底物的生长。突变体实验应能证明戊糖/戊醇利用作为鉴定木葡萄球菌和腐生葡萄球菌的生化特征的价值。可以分离出戊糖阴性的木葡萄球菌突变体,其戊糖/戊醇代谢与腐生葡萄球菌相似,但未能分离出戊糖阳性的木葡萄球菌突变体。这表明木糖和/或阿拉伯糖的降解是木葡萄球菌菌株的特征。戊糖阴性木糖突变体缺乏戊糖异构化活性,但dna - dna杂交研究表明,它们仍然是典型的木糖。野生菌株与突变菌株的dna同源性约为100%,而突变菌株与戊糖阴性的腐生葡萄球菌野生型的dna同源性为35-40%,对应于野生型与腐生葡萄球菌的dna同源性。
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