Unravelling host-pathogen interactions by biofilm infected human wound models

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2023-11-02 DOI:10.1016/j.bioflm.2023.100164
Jana Wächter , Pia K. Vestweber , Viktoria Planz, Maike Windbergs
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Abstract

Approximately 80 % of persistent wound infections are affected by the presence of bacterial biofilms, resulting in a severe clinical challenge associated with prolonged healing periods, increased morbidity, and high healthcare costs. Unfortunately, in vitro models for wound infection research almost exclusively focus on early infection stages with planktonic bacteria. In this study, we present a new approach to emulate biofilm-infected human wounds by three-dimensional human in vitro systems. For this purpose, a matured biofilm consisting of the clinical key wound pathogen Pseudomonas aeruginosa was pre-cultivated on electrospun scaffolds allowing for non-destructive transfer of the matured biofilm to human in vitro wound models. We infected tissue-engineered human in vitro skin models as well as ex vivo human skin explants with the biofilm and analyzed structural tissue characteristics, biofilm growth behavior, and biofilm-tissue interactions. The structural development of biofilms in close proximity to the tissue, resulting in high bacterial burden and in vivo-like morphology, confirmed a manifest wound infection on all tested wound models, validating their applicability for general investigations of biofilm growth and structure. The extent of bacterial colonization of the wound bed, as well as the subsequent changes in molecular composition of skin tissue, were inherently linked to the characteristics of the underlying wound models including their viability and origin. Notably, the immune response observed in viable ex vivo and in vitro models was consistent with previous in vivo reports. While ex vivo models offered greater complexity and closer similarity to the in vivo conditions, in vitro models consistently demonstrated higher reproducibility. As a consequence, when focusing on direct biofilm-skin interactions, the viability of the wound models as well as their advantages and limitations should be aligned to the particular research question of future studies. Altogether, the novel model allows for a systematic investigation of host-pathogen interactions of bacterial biofilms and human wound tissue, also paving the way for development and predictive testing of novel therapeutics to combat biofilm-infected wounds.

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通过生物膜感染人体伤口模型揭示宿主-病原体相互作用
大约80%的持续性伤口感染受到细菌生物膜存在的影响,导致严重的临床挑战,与愈合时间延长、发病率增加和高医疗费用相关。不幸的是,伤口感染的体外模型研究几乎完全集中在浮游细菌的早期感染阶段。在这项研究中,我们提出了一种新的方法,通过三维人体体外系统模拟生物膜感染的人体伤口。为此,在电纺丝支架上预培养一种由临床关键伤口病原体铜绿假单胞菌组成的成熟生物膜,允许将成熟生物膜非破坏性地转移到体外人体伤口模型中。我们用生物膜感染组织工程人体外皮肤模型和离体人皮肤外植体,并分析了组织结构特征、生物膜生长行为以及生物膜与组织的相互作用。生物膜的结构发育靠近组织,导致高细菌负荷和体内样形态,在所有测试的伤口模型上证实了明显的伤口感染,验证了它们对生物膜生长和结构的一般调查的适用性。细菌在伤口床上的定植程度,以及随后皮肤组织分子组成的变化,与潜在伤口模型的特征(包括其生存能力和起源)有着内在的联系。值得注意的是,在活的离体和体外模型中观察到的免疫反应与先前的体内报告一致。虽然离体模型提供了更大的复杂性和更接近体内条件的相似性,但体外模型始终显示出更高的可重复性。因此,当关注直接的生物膜-皮肤相互作用时,伤口模型的可行性以及它们的优势和局限性应该与未来研究的特定研究问题保持一致。总之,新模型允许系统地研究细菌生物膜和人体伤口组织的宿主-病原体相互作用,也为开发和预测新型治疗方法来对抗生物膜感染的伤口铺平了道路。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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