20(S)-Protopanaxadiol from Panax ginseng Induces Apoptosis and Autophagy in Gastric Cancer Cells by Inhibiting Src.

IF 4.8 2区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE American Journal of Chinese Medicine Pub Date : 2023-01-01 DOI:10.1142/S0192415X2350012X

Chaoran Song, Ting Shen, Han Gyung Kim, Weicheng Hu, Jae Youl Cho

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引用次数: 3

Abstract

20(S)-protopanaxadiol (PPD), a metabolite of Panax ginseng, has multiple pharmacological properties. However, the effects of PPD against human gastric cancer have not been elucidated. Our purpose in this study was to investigate if PPD has anticancer effects against human gastric cancer in vitro. Cell viability, migration, clone formation, and invasion were assessed to explore the effects of PPD on cancer cells. PI and annexin V staining as well as immunoblotting were employed to determine if PPD-induced apoptosis and autophagy of MKN1 and MKN45 cells. The target of PPD was identified using immunoblotting, overexpression analysis, and flow cytometric analysis. PPD exhibited significantly suppressed cell viability, migration, colony formation, and invasion. Phosphorylation of Src and its down-stream effectors were inhibited by PPD. PPD-enhanced apoptosis and autophagy in a dose- and time-dependent manner by inhibiting Src. Collectively, our results demonstrate that PPD induces apoptosis and autophagy in gastric cancer cells in vitro by inhibiting Src.

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人参20(S)-原人参二醇通过抑制Src诱导胃癌细胞凋亡和自噬。

20(S)-原人参二醇(PPD)是人参的代谢物，具有多种药理特性。然而，PPD对人胃癌的作用尚未被阐明。本研究的目的是探讨PPD在体外对人胃癌是否有抗癌作用。评估细胞活力、迁移、克隆形成和侵袭，以探讨PPD对癌细胞的影响。采用PI、膜联蛋白V染色和免疫印迹法检测ppd是否诱导MKN1和MKN45细胞凋亡和自噬。通过免疫印迹、过表达分析和流式细胞术分析确定PPD的靶点。PPD显著抑制细胞活力、迁移、集落形成和侵袭。Src及其下游效应物的磷酸化被PPD抑制。ppd通过抑制Src以剂量和时间依赖的方式增强细胞凋亡和自噬。综上所述，我们的研究结果表明PPD通过抑制Src在体外诱导胃癌细胞凋亡和自噬。

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来源期刊

American Journal of Chinese Medicine 医学-全科医学与补充医学

CiteScore

9.90

自引率

8.80%

发文量

159

审稿时长

4.5 months

期刊介绍： The American Journal of Chinese Medicine, which is defined in its broadest sense possible, publishes original articles and essays relating to traditional or ethnomedicine of all cultures. Areas of particular interest include: Basic scientific and clinical research in indigenous medical techniques, therapeutic procedures, medicinal plants, and traditional medical theories and concepts; Multidisciplinary study of medical practice and health care, especially from historical, cultural, public health, and socioeconomic perspectives; International policy implications of comparative studies of medicine in all cultures, including such issues as health in developing countries, affordability and transferability of health-care techniques and concepts; Translating scholarly ancient texts or modern publications on ethnomedicine. The American Journal of Chinese Medicine will consider for publication a broad range of scholarly contributions, including original scientific research papers, review articles, editorial comments, social policy statements, brief news items, bibliographies, research guides, letters to the editors, book reviews, and selected reprints.