Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina).

IF 1.4 4区生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI:10.1615/IntJMedMushrooms.2022046420

Peixin He, Zhuo Chen, Ying Men, Miaomiao Wang, Wensheng Wang, Wei Liu

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引用次数: 2

Abstract

Strain aging has been mainly contributing to the "uncertainty" of Morchella farming. The situation calls for urgent quantitative assessment of strain aging in cultivated Morchella mushrooms. In this paper, systemic senescence of the productive strains of M. eximia, M. importuna, and M. sextelata was achieved through successive subculturing to provide subcultures with different degree of aging for further studies. Then the quantitative assessment of morel strain aging was conducted by activity assay of amylase and xylanase using dinitrosalicylic acid (DNS) method. The results suggested that both activity of amylase and xylanase decreased along with the rise of subculture times. Meanwhile, the correlation between xylanase activity and time of subculturing in the tested morel strains was higher than that of amylase assay. Consequently, assay of amylase and xylanase activity by DNS method can be used in the quantitative assessment of morel strain aging, and assay of xylanase activity is the better alternative. The work will improve the settlement of "uncertainty" in the morel industry and thus be beneficial for stable development of morel farming.

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淀粉酶和木聚糖酶活性测定可定量评价栽培食药用菌菌种老化。

菌种老化是羊肚菌养殖“不确定性”的主要原因。这种情况迫切需要对栽培羊肚菌菌种老化进行定量评估。本文通过连续继代培养，实现了产菌eximia、M. importuna和M. sextelata的系统性衰老，为进一步研究提供了不同程度衰老的继代培养。采用二硝基水杨酸(DNS)法测定淀粉酶和木聚糖酶活性，定量评价菌株老化情况。结果表明，随着继代次数的增加，淀粉酶和木聚糖酶活性均呈下降趋势。同时，木聚糖酶活性与继代时间的相关性高于淀粉酶试验。因此，用DNS法测定淀粉酶和木聚糖酶活性可用于霉菌菌株老化的定量评价，而测定木聚糖酶活性是较好的替代方法。这项工作将有助于解决羊肚菌产业中的“不确定性”，从而有利于羊肚菌养殖的稳定发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International journal of medicinal mushrooms 医学-药学

CiteScore

2.60

自引率

16.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.