METTL14 Promotes Oral Squamous Cell Carcinoma Progression by Regulating the mRNA and m6A Levels of CALD1.

IF 2.1 4区医学 Q3 TOXICOLOGY Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2023-01-01 DOI:10.1615/JEnvironPatholToxicolOncol.2022045134

Ruixue Chen, Suxin Zhang, Hexiang Li, Mengyuan Yang, Yiwen Lu, Xudong Zhang

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Abstract

Oral squamous cell carcinoma (OSCC) still threatens people's daily life. METTL14 is a newly discovered methyltransferase that catalyzes m6A methylation. Hence, this research was carried out to investigate the action mechanism of METTL14 in OSCC. The SCC-4 and UM2 cells, and tumorigenicity assay were utilized to investigate METTL14 roles in vitro and in vivo. Bioinformatic analysis was carried out with the UCSC, TCGA database and The Human Protein Atlas. The gene expression at mRNA and protein levels were measured by qRT-PCR and Western blot. In addition, cell growth and metastasis was analyzed by colony formation and transwell assays. MeRIP assay was performed to test the m6A levels of CALD1. The METTL14 and CALD1 levels were prominently expressed in OSCC cells. METTL14 silencing depleted the cell growth and metastasis. Furthermore, METTL14 silencing depleted the tumor growth in vivo. Additionally, the mRNA and m6A levels of CALD1 were depleted after METTL14 silencing. Overexpressed CALD1 neutralized the si-METTL14 effects in OSCC cells. In conclusion, METTL14 participated in the OSCC progression through modulating the mRNA and m6A levels of CALD1.

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METTL14通过调节CALD1 mRNA和m6A水平促进口腔鳞状细胞癌的进展。

口腔鳞状细胞癌仍然威胁着人们的日常生活。METTL14是一种新发现的催化m6A甲基化的甲基转移酶。因此，本研究旨在探讨METTL14在OSCC中的作用机制。利用SCC-4和UM2细胞及致瘤性实验研究METTL14在体外和体内的作用。生物信息学分析采用UCSC、TCGA数据库和the Human Protein Atlas。采用qRT-PCR和Western blot检测基因在mRNA和蛋白水平上的表达。此外，通过菌落形成和transwell实验分析了细胞的生长和转移。MeRIP法检测CALD1的m6A水平。METTL14和CALD1水平在OSCC细胞中显著表达。METTL14沉默抑制了细胞的生长和转移。此外，METTL14沉默抑制了肿瘤在体内的生长。此外，METTL14沉默后，CALD1的mRNA和m6A水平降低。过表达的CALD1中和了si-METTL14在OSCC细胞中的作用。综上所述，METTL14通过调节CALD1 mRNA和m6A水平参与了OSCC的进展。

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来源期刊

Journal of Environmental Pathology Toxicology and Oncology 医学-毒理学

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Environmental Pathology, Toxicology and Oncology publishes original research and reviews of factors and conditions that affect human and animal carcinogensis. Scientists in various fields of biological research, such as toxicologists, chemists, immunologists, pharmacologists, oncologists, pneumologists, and industrial technologists, will find this journal useful in their research on the interface between the environment, humans, and animals.