CRISPR-mediated iron and folate biofortification in crops: advances and perspectives.

IF 6.5 3区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-04-24 DOI:10.1080/02648725.2023.2205202
Ahamed Khan, Baveesh Pudhuvai, Ankita Shrestha, Ajay Kumar Mishra, Maulin P Shah, Bhupendra Koul, Nrisingha Dey
{"title":"CRISPR-mediated iron and folate biofortification in crops: advances and perspectives.","authors":"Ahamed Khan, Baveesh Pudhuvai, Ankita Shrestha, Ajay Kumar Mishra, Maulin P Shah, Bhupendra Koul, Nrisingha Dey","doi":"10.1080/02648725.2023.2205202","DOIUrl":null,"url":null,"abstract":"<p><p>Micronutrient deficiency conditions, such as anemia, are the most prevalent global health problem due to inadequate iron and folate in dietary sources. Biofortification advancements can propel the rapid amelioration of nutritionally beneficial components in crops that are required to combat the adverse effects of micronutrient deficiencies on human health. To date, several strategies have been proposed to increase micronutrients in plants to improve food quality, but very few approaches have intrigued `clustered regularly interspaced short palindromic repeats' (CRISPR) modules for the enhancement of iron and folate concentration in the edible parts of plants. In this review, we discuss two important approaches to simultaneously enhance the bioavailability of iron and folate concentrations in rice endosperms by utilizing advanced CRISPR-Cas9-based technology. This includes the 'tuning of cis-elements' and 'enhancer re-shuffling' in the regulatory components of genes that play a vital role in iron and folate biosynthesis/transportation pathways. In particular, base-editing and enhancer re-installation in native promoters of selected genes can lead to enhanced accumulation of iron and folate levels in the rice endosperm. The re-distribution of micronutrients in specific plant organs can be made possible using the above-mentioned contemporary approaches. Overall, the present review discusses the possible approaches for synchronized iron and folate biofortification through modification in regulatory gene circuits employing CRISPR-Cas9 technology.</p>","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4138-4168"},"PeriodicalIF":6.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology & Genetic Engineering Reviews","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/02648725.2023.2205202","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/4/24 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Micronutrient deficiency conditions, such as anemia, are the most prevalent global health problem due to inadequate iron and folate in dietary sources. Biofortification advancements can propel the rapid amelioration of nutritionally beneficial components in crops that are required to combat the adverse effects of micronutrient deficiencies on human health. To date, several strategies have been proposed to increase micronutrients in plants to improve food quality, but very few approaches have intrigued `clustered regularly interspaced short palindromic repeats' (CRISPR) modules for the enhancement of iron and folate concentration in the edible parts of plants. In this review, we discuss two important approaches to simultaneously enhance the bioavailability of iron and folate concentrations in rice endosperms by utilizing advanced CRISPR-Cas9-based technology. This includes the 'tuning of cis-elements' and 'enhancer re-shuffling' in the regulatory components of genes that play a vital role in iron and folate biosynthesis/transportation pathways. In particular, base-editing and enhancer re-installation in native promoters of selected genes can lead to enhanced accumulation of iron and folate levels in the rice endosperm. The re-distribution of micronutrients in specific plant organs can be made possible using the above-mentioned contemporary approaches. Overall, the present review discusses the possible approaches for synchronized iron and folate biofortification through modification in regulatory gene circuits employing CRISPR-Cas9 technology.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
CRISPR 介导的作物铁和叶酸生物强化:进展与前景。
由于膳食中铁和叶酸含量不足,微量营养素缺乏症(如贫血)成为全球最普遍的健康问题。生物强化技术的进步可以推动作物中有益营养成分的快速改善,而这些营养成分正是应对微量营养素缺乏症对人类健康的不利影响所必需的。迄今为止,已经提出了几种增加植物中微量营养元素以提高食品质量的策略,但很少有方法利用 "簇状规则间隔短回文重复序列"(CRISPR)模块来提高植物可食用部分中铁和叶酸的浓度。在这篇综述中,我们讨论了利用先进的 CRISPR-Cas9 技术同时提高水稻胚乳中铁和叶酸浓度的生物利用度的两种重要方法。这包括对在铁和叶酸的生物合成/运输途径中发挥重要作用的基因的调控元件进行 "顺式元件调整 "和 "增强子重组"。特别是,在选定基因的原生启动子中进行碱基编辑和增强子重装,可以提高水稻胚乳中铁和叶酸的积累水平。利用上述当代方法,可以实现微量营养元素在特定植物器官中的重新分配。总之,本综述讨论了通过利用 CRISPR-Cas9 技术修改调控基因回路,实现铁和叶酸同步生物强化的可能方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biotechnology & Genetic Engineering Reviews
Biotechnology & Genetic Engineering Reviews BIOTECHNOLOGY & APPLIED MICROBIOLOGY-GENETICS & HEREDITY
CiteScore
6.50
自引率
3.10%
发文量
33
期刊介绍: Biotechnology & Genetic Engineering Reviews publishes major invited review articles covering important developments in industrial, agricultural and medical applications of biotechnology.
期刊最新文献
Application of Shouwu Yizhi prescription in decubation of patients with ischemic stroke. Analysis of non-targeted serum metabolomics in patients with chronic kidney disease and hyperuricemia. Clinical effect of laparoscopic cholecystectomy in the treatment of chronic cholecystitis with gallstones. Evaluating the value of progressive muscle relaxation therapy for patients with lumbar disc herniation after surgery based on a difference-in-differences model. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1