Modified Zhibai Dihuang pill alleviated urinary tract infection induced by extended-spectrum β-lactamase Escherichia coli in rats by regulating biofilm formation.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2023-12-01 DOI:10.1080/13880209.2023.2199786
Kaifa Chen, Yongsheng Zhu, Hongwei Su, Hao Jiang, Xin Liu
{"title":"Modified Zhibai Dihuang pill alleviated urinary tract infection induced by extended-spectrum β-lactamase <i>Escherichia coli</i> in rats by regulating biofilm formation.","authors":"Kaifa Chen, Yongsheng Zhu, Hongwei Su, Hao Jiang, Xin Liu","doi":"10.1080/13880209.2023.2199786","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>Zhibai Dihuang pill (ZD), a traditional Chinese medicine nourishes Yin and reduces internal heat, is believed to have therapeutic effects on urinary tract infections (UTIs).</p><p><strong>Objective: </strong>To explore the effects and mechanism of modified ZD (MZD) on UTI induced by extended-spectrum β-lactamase (ESBLs) <i>Escherichia coli</i>.</p><p><strong>Materials and methods: </strong>Thirty Sprague-Dawley rats were randomly divided into control, model (0.5 mL 1.5 × 10<sup>8</sup> CFU/mL ESBLs <i>E. coli</i>), MZD (20 g/kg MZD), LVFX (0.025 g/kg LVFX), and MZD + LVFX groups (20 g/kg MZD + 0.025 g/kg LVFX), <i>n</i> = 6. After 14 days of treatment, serum biochemical indicators, renal function indicators, bladder and renal histopathology, and urine bacterial counts in rats were determined. Additionally, the effects of MZD on ESBLs <i>E. coli</i> biofilm formation and related gene expression were analyzed.</p><p><strong>Results: </strong>MZD significantly decreased the count of white blood cells (from 13.12 to 9.13), the proportion of neutrophils (from 43.53 to 23.18), C-reactive protein (from 13.21 to 9.71), serum creatinine (from 35.78 to 30.15), and urea nitrogen (from 12.56 to 10.15), relieved the inflammation and fibrosis of bladder and kidney tissues, and reduced the number of bacteria in urine (from 2174 to 559). In addition, MZD inhibited the formation of ESBLs <i>E. coli</i> biofilms (2.04-fold) and decreased the gene expressions of <i>luxS</i>, <i>pfS</i> and <i>ompA</i> (1.41-1.62-fold).</p><p><strong>Discussion and conclusion: </strong>MZD treated ESBLs <i>E. coli-</i>induced UTI inhibited biofilm formation, providing a theoretical basis for the clinical application of MZD. Further study on the clinical effect of MZD may provide a novel therapy option for UTI.</p>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/09/f5/IPHB_61_2199786.PMC10132235.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/13880209.2023.2199786","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

Abstract

Context: Zhibai Dihuang pill (ZD), a traditional Chinese medicine nourishes Yin and reduces internal heat, is believed to have therapeutic effects on urinary tract infections (UTIs).

Objective: To explore the effects and mechanism of modified ZD (MZD) on UTI induced by extended-spectrum β-lactamase (ESBLs) Escherichia coli.

Materials and methods: Thirty Sprague-Dawley rats were randomly divided into control, model (0.5 mL 1.5 × 108 CFU/mL ESBLs E. coli), MZD (20 g/kg MZD), LVFX (0.025 g/kg LVFX), and MZD + LVFX groups (20 g/kg MZD + 0.025 g/kg LVFX), n = 6. After 14 days of treatment, serum biochemical indicators, renal function indicators, bladder and renal histopathology, and urine bacterial counts in rats were determined. Additionally, the effects of MZD on ESBLs E. coli biofilm formation and related gene expression were analyzed.

Results: MZD significantly decreased the count of white blood cells (from 13.12 to 9.13), the proportion of neutrophils (from 43.53 to 23.18), C-reactive protein (from 13.21 to 9.71), serum creatinine (from 35.78 to 30.15), and urea nitrogen (from 12.56 to 10.15), relieved the inflammation and fibrosis of bladder and kidney tissues, and reduced the number of bacteria in urine (from 2174 to 559). In addition, MZD inhibited the formation of ESBLs E. coli biofilms (2.04-fold) and decreased the gene expressions of luxS, pfS and ompA (1.41-1.62-fold).

Discussion and conclusion: MZD treated ESBLs E. coli-induced UTI inhibited biofilm formation, providing a theoretical basis for the clinical application of MZD. Further study on the clinical effect of MZD may provide a novel therapy option for UTI.

Abstract Image

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
栀白地黄丸通过调节生物膜的形成,减轻广谱β-内酰胺酶引起的大鼠尿路感染。
背景:知白地黄丸是一种滋阴降内热的中药,具有治疗尿路感染的作用。材料和方法:30只Sprague-Dawley大鼠随机分为对照组、模型组(0.5 毫升1.5 × 108 CFU/mL ESBLs大肠杆菌)、MZD(20 g/kg MZD)、LVFX(0.025 g/kg LVFX)和MZD + LVFX组(20 g/kg MZD+0.025 g/kg LVFX),n = 6.治疗14天后,测定大鼠血清生化指标、肾功能指标、膀胱和肾脏组织病理学以及尿液细菌计数。此外,还分析了MZD对ESBLs大肠杆菌生物膜形成和相关基因表达的影响。结果:MZD显著降低了白细胞计数(从13.12降至9.13)、中性粒细胞比例(从43.53降至23.18)、C反应蛋白(从13.21降至9.71)、血清肌酐(从35.78降至30.15)和尿素氮(从12.56降至10.15),缓解了膀胱和肾组织的炎症和纤维化,并减少了尿液中的细菌数量(从2174降至559)。此外,MZD抑制ESBLs大肠杆菌生物膜的形成(2.04倍),降低luxS、pfS和ompA的基因表达(1.41-1.62倍)。进一步研究MZD的临床疗效可能为UTI提供一种新的治疗选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊最新文献
Hyperbaric oxygen treatment promotes tendon-bone interface healing in a rabbit model of rotator cuff tears. Oxygen-ozone therapy for myocardial ischemic stroke and cardiovascular disorders. Comparative study on the anti-inflammatory and protective effects of different oxygen therapy regimens on lipopolysaccharide-induced acute lung injury in mice. Heme oxygenase/carbon monoxide system and development of the heart. Hyperbaric oxygen for moderate-to-severe traumatic brain injury: outcomes 5-8 years after injury.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1