Uncovering Gene Expression Profiles of Three Morphologic Mycelium Forms of the Chinese Caterpillar Mushroom Ophiocordyceps sinensis (Ascomycota) Using High-Throughput Sequencing.

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI:10.1615/IntJMedMushrooms.2023047722
Li He, Fang Xie, Xia Ying Xia, Qiang Jun Su, Gang Zhou, Zhao He Chen, Cheng Gang Wang
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Abstract

The asexual form of Ophiocordyceps sinensis has been controversial, but various morphologic mycelium appeared when O. sinensis was cultured under experimental conditions. To explore the generation mechanism of morphologic mycelium, developmental transcriptomes were analyzed from three kinds of mycelium (aerial mycelium, hyphae knot, and substrate mycelium). The results showed that diameter and morphology of these three kinds of mycelium were obviously different. KEGG functional enrichment analysis showed that the differential expressed genes (DEGs) of substrate mycelium were enriched in ribosomes and peroxisomes, indicating that prophase culture was rich in nutrients and the metabolism of substrate mycelium cells was vigorous in the stage of nutrient absorption. The up-DEGs of hyphae knot were mainly enriched in the oxidative phosphorylation pathway, indicating that oxidative phosphorylation was the main energy source for mycelium formation in the stage of nutrient accumulation and reproductive transformation. The up-DEGs of aerial mycelium were mainly enriched in the synthesis and degradation pathways of valine, leucine, and isoleucine, suggesting that the occurrence of aerial mycelium was related to amino acid metabolism at the later stage of culture, and nutritional stress accelerated the reproduction of asexual spores. In addition, the important roles of mycelium formation related genes were verified by combined analysis of qRT-PCR and transcriptome sequencing. Collectively, this study will provide theoretical guidance for inhibiting the occurrence of aerogenous mycelium and promoting the development of mycelium into pinhead primordia in the culture of O. sinensis in the future.

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利用高通量测序技术揭示冬虫夏草(Ophiocordyceps sinensis (Ascomycota))三种形态菌丝体的基因表达谱
关于冬虫夏草的无性形态一直存在争议,但在实验条件下,冬虫夏草出现了多种形态的菌丝体。为了探讨形态菌丝的产生机制,对三种菌丝(气生菌丝、菌丝结和底生菌丝)的发育转录组进行了分析。结果表明,这三种菌丝体的直径和形态有明显差异。KEGG功能富集分析显示,底物菌丝的差异表达基因(DEGs)在核糖体和过氧化物酶体中富集,说明前期培养营养丰富,底物菌丝细胞在营养吸收阶段代谢旺盛。菌丝结的上deg主要富集于氧化磷酸化途径,说明在营养积累和生殖转化阶段,氧化磷酸化是菌丝形成的主要能量来源。空中菌丝体的上升基因主要富集于缬氨酸、亮氨酸和异亮氨酸的合成和降解途径,说明空中菌丝体的发生与培养后期氨基酸代谢有关,营养胁迫加速了无性孢子的繁殖。此外,通过qRT-PCR和转录组测序联合分析,验证了菌丝形成相关基因的重要作用。综上所述,本研究将为今后在中华玉螺培养中抑制气生菌丝体的发生,促进菌丝体向针尖原基的发育提供理论指导。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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