Evaluation of the Sysmex UF-4000i urine analyzer as a screening test to rule out urinary tract infection and reduce urine cultures.

IF 0.4 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY Annales de biologie clinique Pub Date : 2023-05-16 DOI:10.1684/abc.2023.1797
Assiya El Kettani, Samy Housbane, Fatima Wakit, Karima Arioua Mikou, Houria Belabbes, Khalid Zerouali
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Abstract

Introduction: Urinary tract infection (UTI) diagnosis by urine culture is time- and labor- consuming. In the Ibn Rochd microbiology laboratory, up to 70% of urine culture samples yield no growth or insignificant growth.

Objective: To evaluate the new generation of Sysmex UF-4000i fluorescence flow cytometry analyzer with a blue semiconducting laser as a method to rule out negative urine samples for UTI, in comparison of urine culture.

Material and methods: Flow cytometry and microbiological analysis were performed on 502 urine samples included in the study. We used ROC analysis to determine cutoff points at which optimal sensitivity and specificity are achieved for clinical use.

Results: Our results showed that bacteria count at a cut-off of 100/μL, and/or the leucocytes count ≥ 45/μL are the optimal indicator for positive culture results. At these cut off, bacteria sensitivity (SE), specificity (SP), Positive predictive value (PPV) and negative predictive value (NPV) were 97,3%, 95%, 87,8% and 98,8% respectively. For leucocytes, SE, SP, PPV and NPV were 99,1%, 95,8%, 88,6% and 99,7% respectively.

Discussion and conclusion: The bacterial and leucocytes counts generated by UF-4000i analysis may be useful in our context as a rapid screening to exclude UTI by reducing about 70% of urines cultures and then workload. Nevertheless, further validation is needed for different patient groups especially with urological disease or immunocompromised patients.

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评价Sysmex UF-4000i尿液分析仪作为排除尿路感染和减少尿培养的筛选试验。
导读:尿路感染(UTI)的尿培养诊断费时费力。在伊本罗氏微生物实验室,高达70%的尿液培养样本没有生长或生长不明显。目的:评价新一代Sysmex UF-4000i蓝光半导体激光荧光流式细胞仪在尿液培养阴性尿路感染排除中的应用价值。材料和方法:对纳入研究的502份尿液样本进行了流式细胞术和微生物学分析。我们使用ROC分析来确定达到临床使用的最佳灵敏度和特异性的截止点。结果:细菌数≥100/μL和/或白细胞数≥45/μL为最佳阳性培养指标。细菌敏感性(SE)、特异性(SP)、阳性预测值(PPV)和阴性预测值(NPV)分别为97、3%、95%、87、8%和98、8%。白细胞SE、SP、PPV和NPV分别为99、1%、95、8%、88、6%和99、7%。讨论和结论:UF-4000i分析产生的细菌和白细胞计数可能在我们的背景下有用,通过减少约70%的尿培养和工作量,作为排除尿路感染的快速筛查。然而,需要对不同的患者群体进行进一步的验证,特别是泌尿系统疾病或免疫功能低下的患者。
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来源期刊
Annales de biologie clinique
Annales de biologie clinique 医学-医学:研究与实验
CiteScore
0.80
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Multidisciplinary information with direct relevance to everyday practice Annales de Biologie Clinique, the official journal of the French Society of Clinical Biology (SFBC), supports biologists in areas including continuing education, laboratory accreditation and technique validation. With original articles, abstracts and accounts of everyday practice, the journal provides details of advances in knowledge, techniques and equipment, as well as a forum for discussion open to the entire community.
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