Mammalian cell and tissue imaging using Raman and coherent Raman microscopy.

IF 7.9 Q1 Medicine Wiley Interdisciplinary Reviews-Systems Biology and Medicine Pub Date : 2020-11-01 Epub Date: 2020-07-19 DOI:10.1002/wsbm.1501
Anthony A Fung, Lingyan Shi
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引用次数: 0

Abstract

Direct imaging of metabolism in cells or multicellular organisms is important for understanding many biological processes. Raman scattering (RS) microscopy, particularly, coherent Raman scattering (CRS) such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), has emerged as a powerful platform for cellular imaging due to its high chemical selectivity, sensitivity, and imaging speed. RS microscopy has been extensively used for the identification of subcellular structures, metabolic observation, and phenotypic characterization. Conjugating RS modalities with other techniques such as fluorescence or infrared (IR) spectroscopy, flow cytometry, and RNA-sequencing can further extend the applications of RS imaging in microbiology, system biology, neurology, tumor biology and more. Here we overview RS modalities and techniques for mammalian cell and tissue imaging, with a focus on the advances and applications of CARS and SRS microscopy, for a better understanding of the metabolism and dynamics of lipids, protein, glucose, and nucleic acids in mammalian cells and tissues. This article is categorized under: Laboratory Methods and Technologies > Imaging Biological Mechanisms > Metabolism Analytical and Computational Methods > Analytical Methods.

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利用拉曼显微镜和相干拉曼显微镜进行哺乳动物细胞和组织成像。
对细胞或多细胞生物体内的新陈代谢进行直接成像对于了解许多生物过程非常重要。拉曼散射(RS)显微镜,尤其是相干拉曼散射(CRS),如相干反斯托克斯拉曼散射(CARS)和受激拉曼散射(SRS),因其化学选择性高、灵敏度高和成像速度快,已成为细胞成像的强大平台。RS 显微镜已被广泛用于亚细胞结构鉴定、代谢观察和表型特征描述。将 RS 模式与荧光或红外(IR)光谱、流式细胞仪和 RNA 序列测定等其他技术相结合,可进一步扩展 RS 成像在微生物学、系统生物学、神经学、肿瘤生物学等领域的应用。在此,我们将概述用于哺乳动物细胞和组织成像的 RS 模式和技术,重点介绍 CARS 和 SRS 显微镜的进展和应用,以便更好地了解哺乳动物细胞和组织中脂类、蛋白质、葡萄糖和核酸的代谢和动态。本文归类于实验室方法和技术 > 生物机制成像 > 新陈代谢 分析和计算方法 > 分析方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
18.40
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Journal Name:Wiley Interdisciplinary Reviews-Systems Biology and Medicine Focus: Strong interdisciplinary focus Serves as an encyclopedic reference for systems biology research Conceptual Framework: Systems biology asserts the study of organisms as hierarchical systems or networks Individual biological components interact in complex ways within these systems Article Coverage: Discusses biology, methods, and models Spans systems from a few molecules to whole species Topical Coverage: Developmental Biology Physiology Biological Mechanisms Models of Systems, Properties, and Processes Laboratory Methods and Technologies Translational, Genomic, and Systems Medicine
期刊最新文献
Tools for computational analysis of moving boundary problems in cellular mechanobiology. Cellular reprogramming: Mathematics meets medicine. Thermoregulation: A journey from physiology to computational models and the intensive care unit. Mammalian cell and tissue imaging using Raman and coherent Raman microscopy. Computational models to explore the complexity of the epithelial to mesenchymal transition in cancer.
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