SARS-CoV-2 strain-specific anti-spike IgG ELISA utilizing spike protein produced by silkworms.

Q3 Medicine Human Antibodies Pub Date : 2023-01-01 DOI:10.3233/HAB-230006

Takeyuki Goto, Tomoki Sasaki, Yong Chong, Masahiro Taniguchi, Jae Man Lee, Akitsu Masuda, Takeru Ebihara, Kenichiro Shiraishi, Naoki Tani, Akiko Yonekawa, Kei Gondo, Hiroyuki Kuwano, Nobuyuki Shimono, Hideyuki Ikematsu, Koichi Akashi, Takahiro Kusakabe

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Abstract

Background: A cost-effective and eco-friendly method is needed for the assessment of humoral immunity against SARS-CoV-2 in large populations.

Objective: We investigated the performance of an ELISA that uses silkworm-produced proteins to quantify the strain-specific anti-Spike IgG (anti-S IgG) titer.

Methods: The OD values for the anti-His-tag antibody, a standard material of ELISA quantification, were measured. Correlations between the ELISA for each strain and the Abbott SARS-CoV-2 IgG II Quant assay for the wild type were evaluated with serum samples from nine participants with various infection and vaccination statuses.

Results: Linear dose-responses were confirmed by high coefficients of determination: 0.994, 0.994, and 0.996 for the wild-type, Delta, and Omicron (BA.1) strain assays, respectively. The coefficient of determination for the wild-type and Delta strain assays was high at 0.959 and 0.892, respectively, while the Omicron strain assay had a relatively low value of 0.563. Booster vaccinees showed similar or higher titers against all strains compared to infected persons without vaccination. The Omicron-infected persons without vaccination had lower antibody titers against wild type than did the vaccinated persons.

Conclusions: This study provides data indicating that the ELISA with silkworm-produced proteins makes it possible to discriminate and quantify the strain-specific anti-S IgG antibody induced by vaccination or infection.

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利用蚕产生的刺突蛋白的严重急性呼吸系统综合征冠状病毒2株特异性抗刺突IgG ELISA。

背景：需要一种经济高效、环保的方法来评估大规模人群对严重急性呼吸系统综合征冠状病毒2型的体液免疫。目的：我们研究了利用家蚕产生的蛋白质来定量菌株特异性抗刺突IgG（抗S IgG）滴度的ELISA的性能。方法：测定ELISA定量标准物质抗His-tag抗体的OD值。用9名具有不同感染和疫苗接种状态的参与者的血清样本评估了每种毒株的ELISA和野生型Abbott SARS-CoV-2 IgG II Quant测定之间的相关性。结果：野生型、德尔塔和奥密克戎（BA.1）菌株测定的线性剂量反应得到了高确定系数的证实：分别为0.994、0.994和0.996。野生型和德尔塔毒株测定的测定系数分别为0.959和0.892，而奥密克戎毒株测定的值相对较低，为0.563。与未接种疫苗的感染者相比，加强疫苗接种者对所有菌株的滴度相似或更高。未接种疫苗的奥密克戎感染者对野生型的抗体滴度低于接种疫苗的人。结论：本研究提供的数据表明，用家蚕产生的蛋白质进行ELISA可以区分和定量由疫苗接种或感染诱导的菌株特异性抗S IgG抗体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.