Preparation of Zinc Oxide Nanoparticles Assisted by Okra Mucilage and Evaluation of its Biological Activities.

Q3 Pharmacology, Toxicology and Pharmaceutics Current drug discovery technologies Pub Date : 2023-01-01 DOI:10.2174/1570163820666221201090006

Pegah Shakib, Seyedeh Zahra Mirzaei, Hamed Esmaeil Lashgarian, Reza Saki, Gholamreza Goudarzi, Sarah Alsallameh, Abdolrazagh Marzban, Kourosh Cheraghipour

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Abstract

Background: In this study, zinc oxide nanoparticles (ZnO-NPs) were biologically synthesized from Abelmoschus esculentus L. (Okra) mucilage fraction (OM).

Methods: Analytical techniques were employed to study the formation and properties of OM-ZnO NPs, including their morphology, shape, size distribution, and surface charges. Additionally, OM-ZnO NPs were assessed for their antimicrobial, antioxidant, and cytotoxic properties.

Results: UV-visible spectroscopy confirmed the formation of OM-ZnO NPs, evident by the appearance of an SPR peak at 368.8 nm. The FTIR spectroscopy demonstrated that OM functional groups contribute to the formation and stability of the NPs. Micrographs from TEM and SEM showed that OM-ZnO NPs ranged from 15-40 nm in diameter, whereas hydrodynamic diameter and surface charge values obtained from Zeta and DLS were 72.8 nm and 14.6 mv, respectively. XRD analysis indicated the OM-ZnO NPs were crystalline with a wurtzite structure and a crystallite size of 27.3 nm, while EDX revealed a zinc: oxygen ratio of 67.5:34. Further, the OM-ZnO NPs demonstrated significant antimicrobial activity in response to different types of bacteria. In the antioxidant assay, the OM-ZnO NPs scavenged DPPH with 68.6% of the efficiency of ascorbic acid (100%).

Conclusion: The present study demonstrated the cytotoxic efficacy of MO-ZnO NPs against MCF7 cells with an IC50 of 43.99 μg/ml. Overall, the green synthesis of ZnO NPs by OM was successful for many biological applications, such as antimicrobial, antioxidant, and anticancer. Moreover, OM-ZnO NPs can be applied as a biologically-derived nanotherapeutic agent.

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秋葵黏液辅助氧化锌纳米颗粒的制备及其生物活性评价。

背景:以秋葵(Abelmoschus esculentus L.，秋葵)粘液部分(OM)为原料，生物合成氧化锌纳米颗粒(ZnO-NPs)。方法:采用分析技术研究OM-ZnO纳米粒子的形成和性质，包括形貌、形状、尺寸分布和表面电荷。此外，对OM-ZnO NPs的抗菌、抗氧化和细胞毒性进行了评估。结果:紫外可见光谱证实了OM-ZnO NPs的形成，在368.8 nm处出现SPR峰。FTIR光谱分析表明，OM官能团参与了NPs的形成和稳定性。TEM和SEM的显微图显示，OM-ZnO纳米粒子的直径在15 ~ 40 nm之间，而Zeta和DLS的水动力直径和表面电荷值分别为72.8 nm和14.6 mv。XRD分析表明，OM-ZnO纳米粒子呈纤锌矿结构，晶粒尺寸为27.3 nm, EDX分析表明其锌氧比为67.5:34。此外，OM-ZnO NPs对不同类型的细菌表现出显著的抗菌活性。在抗氧化实验中，OM-ZnO NPs清除DPPH的效率为抗坏血酸的68.6%(100%)。结论:MO-ZnO NPs对MCF7细胞具有细胞毒作用，IC50为43.99 μg/ml。综上所述，OM绿色合成ZnO NPs在抗菌、抗氧化和抗癌等生物领域的应用是成功的。此外，OM-ZnO纳米粒子可以作为生物来源的纳米治疗剂。

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来源期刊

Current drug discovery technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

3.70

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0.00%

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期刊介绍： Due to the plethora of new approaches being used in modern drug discovery by the pharmaceutical industry, Current Drug Discovery Technologies has been established to provide comprehensive overviews of all the major modern techniques and technologies used in drug design and discovery. The journal is the forum for publishing both original research papers and reviews describing novel approaches and cutting edge technologies used in all stages of drug discovery. The journal addresses the multidimensional challenges of drug discovery science including integration issues of the drug discovery process.