Antibacterial Activity of Culinary-Medicinal Polypore Mushroom Lentinus tigrinus (Agaricomycetes).

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI:10.1615/IntJMedMushrooms.2023048172
Yasaman Yazdi, Mohammad Reza Rezaei, Hamid R Pourianfar
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Abstract

Medicinal mushrooms belonging to Lentinus spp. exhibit significant antibacterial activities, but little attention has been paid to their efficacy against the food-borne pathogen, Bacillus cereus. The present study for the first time quantitatively evaluated the antibacterial activity of different extracts from fruiting bodies of a well-authenticated Iranian native strain of medicinal mushroom, Lentinus tigrinus, against Gram-positive spore-forming bacterium B. cereus. The findings revealed that the acetone extract inhibited the growth of B. cereus at concentrations as low as 31.25 μg/ML, while it had no effect against Escherichia coli and Staphylococcus aureus even at 10,000 μg/ML. The rest of the bacteria were also susceptible to the acetone extract at concentrations greater than 5 mg/ML. Antibacterial activities of the methanol-ethyl acetate extract and the hot water extract were significantly weaker than that of the acetone extract, which contained high amounts of total phenols (5.83 ± 0.08 mg GAE/g, dw), while Fourier transform infrared spectroscopy (FT-IR) confirmed the presence of functional groups, such as hydroxyl, carbonyl, amide, and amine. Further studies by scanning electron microscopy (SEM) confirmed obvious changes in the morphology of B. cereus in response to the acetone extract of L. tigrinus. This study may suggest that L. tigrinus could be a good natural source for isolating and purifying antibacterial compounds against B. cereus.

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烹饪药用多孔蘑菇的抗菌活性。
香菇属药用蘑菇具有显著的抗菌活性,但对其对抗食源性病原体蜡样芽孢杆菌的功效却鲜有关注。本研究首次定量评估了经鉴定的伊朗本土药用蘑菇香菇子实体不同提取物对革兰氏阳性孢子形成菌蜡样芽孢杆菌的抗菌活性。研究结果表明,丙酮提取物在低至31.25μg/ML的浓度下抑制蜡样芽孢杆菌的生长,而即使在10000μg/ML下也对大肠杆菌和金黄色葡萄球菌没有影响。其余细菌也对浓度大于5mg/ML的丙酮提取物敏感。甲醇-乙酸乙酯提取物和热水提取物的抗菌活性明显弱于丙酮提取物,丙酮提取物含有大量的总酚(5.83±0.08 mg GAE/g,dw),而傅立叶变换红外光谱(FT-IR)证实了官能团的存在,如羟基、羰基、酰胺和胺。通过扫描电子显微镜(SEM)的进一步研究证实,蜡状芽孢杆菌的形态对虎耳球虫丙酮提取物的反应发生了明显变化。本研究可能表明,老虎乳杆菌是分离纯化蜡样芽孢杆菌抗菌化合物的良好天然来源。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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