Extraction, Characterization, and In Vitro Hypoglycemic Activity of a Neutral Polysaccharide from the New Medicinal Mushroom Cantharellus yunnanensis (Agaricomycetes).

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI:10.1615/IntJMedMushrooms.2023049072
Zhang-Chao Pan, Yu-Zhuo Zhang, Zhi-Qun Liang, Yong Wang, Nian-Kai Zeng
{"title":"Extraction, Characterization, and In Vitro Hypoglycemic Activity of a Neutral Polysaccharide from the New Medicinal Mushroom Cantharellus yunnanensis (Agaricomycetes).","authors":"Zhang-Chao Pan,&nbsp;Yu-Zhuo Zhang,&nbsp;Zhi-Qun Liang,&nbsp;Yong Wang,&nbsp;Nian-Kai Zeng","doi":"10.1615/IntJMedMushrooms.2023049072","DOIUrl":null,"url":null,"abstract":"<p><p>Polysaccharides serve as promising ingredients for health-beneficial functional foods, while there were no investigations into the structural characterizations and bioactivities of an edible mushroom Cantharellus yunnanensis. In the study, crude polysaccharides from this mushroom were extracted by hot water and isolated by ethanol precipitation. Then, a neutral polysaccharide (named CY-1) was purified from the crude polysaccharide by deproteinization with Sevag reagent, decolorization with a kind of macroporous adsorption resin SP-825, DEAE-52 cellulose column chromatography and dialysis. The physicochemical properties of CY-1 were characterized by UV, IR, SEM, NMR, and HPLC analyses. Structural characterizations revealed that CY-1 is a homogeneous heteropolysaccharide with an average molecular weight of 3.06 × 104 Da. CY-1 exhibited a honeycomb structure with an irregular branching shape, and it was composed of mannose, glucose, fucose, xylose, arabinose, galactose, rhamnose, and glucuronic acid, with molar fractions of 54.83%, 25.11%, 10.28% 4.53%, 2.12%, 1.64%, 0.83%, and 0.64%, respectively. In vitro hypoglycemic tests showed that CY-1 had an inhibitory effect on α-glucosidase. In addition, CY-1 of 160 µg/mL promoted glucose consumption in normal HepG2 cells. These results suggested that CY-1 may be a potential hypoglycemic agent.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of medicinal mushrooms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1615/IntJMedMushrooms.2023049072","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Polysaccharides serve as promising ingredients for health-beneficial functional foods, while there were no investigations into the structural characterizations and bioactivities of an edible mushroom Cantharellus yunnanensis. In the study, crude polysaccharides from this mushroom were extracted by hot water and isolated by ethanol precipitation. Then, a neutral polysaccharide (named CY-1) was purified from the crude polysaccharide by deproteinization with Sevag reagent, decolorization with a kind of macroporous adsorption resin SP-825, DEAE-52 cellulose column chromatography and dialysis. The physicochemical properties of CY-1 were characterized by UV, IR, SEM, NMR, and HPLC analyses. Structural characterizations revealed that CY-1 is a homogeneous heteropolysaccharide with an average molecular weight of 3.06 × 104 Da. CY-1 exhibited a honeycomb structure with an irregular branching shape, and it was composed of mannose, glucose, fucose, xylose, arabinose, galactose, rhamnose, and glucuronic acid, with molar fractions of 54.83%, 25.11%, 10.28% 4.53%, 2.12%, 1.64%, 0.83%, and 0.64%, respectively. In vitro hypoglycemic tests showed that CY-1 had an inhibitory effect on α-glucosidase. In addition, CY-1 of 160 µg/mL promoted glucose consumption in normal HepG2 cells. These results suggested that CY-1 may be a potential hypoglycemic agent.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
新型药用蘑菇云南Cantharellus yunnanensis中性多糖的提取、表征及体外降糖活性研究。
多糖是一种很有前景的保健功能食品原料,但对云南Cantharellus yunnanensis食用菌的结构特征和生物活性研究尚不深入。本研究采用热水提取粗多糖,乙醇沉淀法分离粗多糖。然后,通过Sevag试剂脱蛋白、SP-825大孔吸附树脂脱色、DEAE-52纤维素柱层析,从粗多糖中纯化出中性多糖CY-1。CY-1的理化性质通过紫外、红外、扫描电镜、核磁共振、高效液相色谱等分析表征。结构表征表明CY-1是一种平均分子量为3.06 × 104 Da的均质杂多糖。CY-1呈不规则分枝状的蜂窝状结构,由甘露糖、葡萄糖、焦糖、木糖、阿拉伯糖、半乳糖、鼠李糖和葡萄糖醛酸组成,摩尔分数分别为54.83%、25.11%、10.28%、4.53%、2.12%、1.64%、0.83%和0.64%。体外降糖实验表明CY-1对α-葡萄糖苷酶有抑制作用。160µg/mL CY-1能促进正常HepG2细胞的葡萄糖消耗。这些结果提示CY-1可能是一种潜在的降糖药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
期刊最新文献
Methanolic Extract of Morchella esculenta (Ascomycota), Prevents Chemotherapy Related Cardiotoxicity in Tumour Bearing Mice Optimization of Mycelial Growth Parameters in Lion’s Mane Edible Medicinal Mushroom Hericium erinaceus (Agaricomycetes) by Supplementation of Growth Hormones Hot Water Extract of Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes) Fruiting Body Activates Myeloid Dendritic Cells through a Dectin-1-Mediated Pathway Enhancement of Cordycepin Production Through Liquid Static Fermentation of Caterpillar Medicinal Mushroom Cordycep militaris (Ascomycetes) Efficacy of Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Supplementation on Psychological Stress and Selective Fitness Profile Parameters in Female College Students in West Bengal, India
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1