动力学的omega-3脂肪酸转移到牛奶不同的脂肪酸和奶牛的泌乳阶段

Natalie L. Urrutia , Michel Baldin , Samantha R. Egolf , Rachel E. Walker , Yun Ying , Michael H. Green , Kevin J. Harvatine
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引用次数: 0

摘要

脂肪酸(FA)在转运效率和代谢分配方面存在差异,泌乳奶牛为研究FA转运动力学提供了一个强有力的模型。目的是比较n-3多不饱和脂肪酸(PUFA)通过血浆和进入牛奶的动力学。在第一个实验中,10头经瘤胃插管的荷斯坦奶牛被用于7天周期的交叉设计。每隔6小时给奶牛挤奶,腹部处理提供单剂量的80.1克游离FA形式的α-亚麻酸(ALA-FFA)或45.5克EPA和32.9克DHA(LCn3-FFA)。ALA-FFA将n-3 PUFA转移到牛奶中比LCn3 FFA高出近50%(团块的48.2%和32.7%),并且符合双指数模型。n-3 PUFA的快速转运(假设直接通过乳糜微粒)在ALA-FFA中几乎是LCn3-FFA的两倍,随后的慢速转运(假设通过组织循环间接转移)在LCn3-FF中比ALA-FFA高2.5倍以上。血浆分析显示,LCn3-FFA富含磷脂和胆固醇酯,清除缓慢。在第二个实验中,4头奶牛在未哺乳期和哺乳期第10、55和225天左右接受了ALA、EPA和DHA制剂的混合物。ALA在不同泌乳阶段转移到乳汁中没有差异,但与泌乳中期和晚期相比,早期的DHA含量较低。总之,饮食中的ALA可以快速有效地转移到奶牛的乳汁中,而EPA和DHA则可以快速掺入乳腺无法获得的血浆或组织成分中。这表明n-3 PUFA的运输和分配存在明显差异,最终影响组织和细胞器的富集,并影响有效剂量。
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Kinetics of omega-3 fatty acid transfer to milk differs between fatty acids and stage of lactation in dairy cows

Fatty acids (FA) differ in their transfer efficiencies and metabolic partitioning and lactating cows provide a robust model to investigate kinetics of FA transport. The objective was to compare kinetics of n-3 polyunsaturated FA (PUFA) trafficking through plasma and into milk. In the first experiment, ten ruminally cannulated multiparous Holstein cows were used in a crossover design with 7 d periods. Cows were milked at 6 h intervals and abomasal treatments provided a single dose of 80.1 g of α-linolenic acid as free FA (ALA-FFA) or 45.5 g EPA and 32.9 g DHA (LCn3-FFA). Transfer of n-3 PUFA to milk was nearly 50% higher for ALA-FFA than LCn3-FFA (48.2 and 32.7% of the bolus) and fit a bi-exponential model. Rapid transport of n-3 PUFA, assumed to be directly through chylomicrons, was nearly twice as high in ALA-FFA than LCn3-FFA and the subsequent slow transport, assumed to be indirect transfer through tissue recycling, was over 2.5-fold higher in LCn3-FFA than in ALA-FFA. Plasma analysis revealed LCn3-FFA enriched phospholipids and cholesterol esters, which had a slow clearance. In the second experiment, 4 cows received a bolus of a mixture of ALA, EPA, and DHA prepartum while not lactating and around d 10, 55, and 225 of lactation. Transfer of ALA to milk did not differ between stages of lactation, but DHA was lower in early compared to mid and late lactation. In conclusion, dietary ALA is rapidly and efficiently transferred to milk in cows while EPA and DHA are rapidly incorporated into plasma or tissue fractions not available to the mammary gland. This demonstrates clear differences in trafficking and partitioning of n-3 PUFA that ultimately impact tissue and organelle enrichment with implications for effective doses.

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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
发文量
0
审稿时长
64 days
期刊最新文献
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