毛茛(Trachystemon orientalis)唐是迷迭香酸的重要来源:生物活性和高效液相色谱分析。

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Turkish Journal of Pharmaceutical Sciences Pub Date : 2023-07-07 DOI:10.4274/tjps.galenos.2022.14265
Burak Bıyık, Sezen Yılmaz Sarıaltın, Alper Gökbulut, Tülay Çoban, Maksut Çoşkun
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引用次数: 2

摘要

目的:毛蕊草(Trachystemon orientalis)唐,在基耶语中俗称为“kaldırık”,是一种属于琉璃苣科的可食用植物。这种植物因其多种治疗作用已在传统医学中使用多年。植物的功效和化学成分可能因其部位、年龄和提取溶剂而异。因此,本研究旨在明确不同季节采集的东方桦幼嫩期和成熟期各部位和提取物的生物活性,并探讨产生这些生物效应的主要成分。材料与方法:采用不同季节采集的植物材料,采自云南西北地区。研究了2,2′-氮唑-(3-乙基苯并噻唑-6-磺酸)(ABTS)和2,2-二苯基-1-吡啶酰肼(DPPH)自由基清除能力,以评价其抗自由基和抗氧化能力。采用人红细胞膜稳定法对提取物的抗炎活性进行了测试。采用Folin-Ciocalteu试验测定总酚含量。反相高效液相色谱-光电二极管阵列检测器(RP-HPLC-PDA)分析。结果:与对照相比,甲醇和水提液均表现出明显的自由基清除和抗炎活性(p)结论:本研究首次发现迷迭香酸在东洋中草药和根中存在。东方红的植物化学成分和有效生物活性解释了其传统用途,并表明其在制药工业中的应用潜力巨大。
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Trachystemon orientalis (L.) G. Don as a Valuable Source of Rosmarinic Acid: Biological Activities and HPLC Profiles.

Objectives: Trachystemon orientalis (L.) G. Don, colloquially known in Türkiye as "kaldırık", is an edible plant belonging to the Boraginaceae. This plant has been practiced in traditional medicine for many years for its various therapeutic benefits. The effectiveness and chemical composition of plants can vary depending on their parts, age, and extraction solvent. Therefore, the current study aimed to define the biological activities of various parts and extracts of T. orientalis, which were collected in distinct seasons as young and mature, and investigate the main component responsible for these biological effects.

Material and methods: Plant material was collected in different seasons from the northwest of Türkiye. 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities were investigated to assess antiradical and antioxidant potential of the extracts. Anti-inflammatory activity of the extracts was also tested using human red blood cell membrane stabilizing method. Folin-Ciocalteu test was conducted to determine the total phenolic content. Reverse phase-high performance liquid chromatography-photodiode array detector (RP-HPLC-PDA) analysis was performed.

Results: Both methanol and aqueous extracts exhibited significant radical scavenging and anti-inflammatory activities compared with control (p<0.05). The highest percentage of inhibition on ABTS and DPPH free radicals was obtained in aqueous extracts of the mature herbs and roots, respectively. Methanol extracts of the mature roots and herbs exhibited the strongest anti-inflammatory capacity. Rosmarinic acid possessed a much higher antioxidant and anti-inflammatory effect than the reference compounds used in each assay in our study. High rosmarinic acid content of the extracts suggests that the compound responsible for the great biological activity potential is rosmarinic acid.

Conclusion: To the best of our knowledge, the presence of rosmarinic acid in herbs and roots of T. orientalis was shown for the first time in our present study. Phytochemical composition and effective biological activities of T. orientalis explain its traditional use and indicate its significant potential in pharmaceutical industry applications.

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CiteScore
3.60
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5.90%
发文量
79
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