体外评估米诺环素电纺纳米纤维膜上 BMSCs 的早期增殖。

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2024-01-01 DOI:10.3233/BME-230002
Quan Liu, Xiao Ma, Yanchen Pei, Wendan Cheng, Zhengwei Wu
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引用次数: 0

摘要

背景:电纺纳米纤维可模拟宿主骨的天然细胞外基质(ECM),而米诺环素(MINO)是一种广谱四环素类抗生素,具有多种非抗生素生物效应,可促进体外和体内成骨:本研究旨在构建一种负载MINO的聚乳酸(PLA)电纺纳米纤维膜,以增强骨髓间充质干细胞(BMSCs)的粘附和增殖,用于早期临床治疗:通过扫描电子显微镜(SEM)、傅立叶变换红外光谱(FTIR)和体外药物释放研究对 MINO-PLA 膜进行了表征。同时还研究了其抗菌能力。此外,还进行了体外细胞增殖实验,以验证负载 MINO 的聚乳酸电纺纳米纤维膜是否能增强 BMSCs 的粘附和增殖:分析药物释放和细胞生长结果发现,只有有效浓度的MINO-PLA才能在短期内促进BMSCs的生长。这与 MINO-PLA 的药物释放率和 MINO 的初始浓度有关:本研究表明,通过控制电纺聚乳酸中 MINO 的浓度和释放率,BMSCs 可以在其上增殖,本研究制成了一种新的骨修复材料。
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In vitro evaluation of BMSCs early proliferation on minocycline-loaded electrospun nanofibers membrane.

Background: Electrospun nanofibers could simulate the natural extracellular matrix (ECM) of the host bone, while minocycline (MINO) is a broad-spectrum tetracycline antibiotic which has been found to have multiple non-antibiotics biological effects that promotes osteogenesis in vitro and in vivo.

Objective: The present study aims at constructing a polylactic acid (PLA) electrospun nanofiber membrane loaded with MINO to enhance Bone marrow mesenchymal stem cells (BMSCs) adhesion and proliferation for early clinical treatment.

Methods: The MINO-PLA membrane were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and in vitro drug release study. The antibacterial ability was also investigated. In addition, in vitro cellular proliferation experiment was performed to verify whether the PLA electrospun nanofibers membrane loaded with MINO enhance BMSCs adhesion and proliferation.

Results: Analyzing the drug release and cell growth results, it was found that only the effective concentration of MINO-PLA could help the growth of BMSCs in the short term. This is related to the drug release rate of MINO-PLA and the initial concentration of MINO.

Conclusion: This study shows that by controlling the concentration and release rate of MINO with electrospinning PLA, BMSCs could proliferate on it, and a new bone repair material had been made in this study.

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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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