{"title":"大香菇蘑菇:分子鉴定、体外抗糖尿病、抗肥胖和细胞毒性评估。","authors":"Oyindamola Olajumoke Abiodun, Adenike Martha Alege, Precious Ulonnam Ezurike, Abraham Nkumah, Oluwatosin Adelowo, Tolulope Aderinola Oke","doi":"10.4274/tjps.galenos.2021.72798","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Mushrooms are fungi with nutritional and health benefits. <i>Lentinus squarrosulus</i> Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the <i>in vitro</i> anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom.</p><p><strong>Materials and methods: </strong><i>L. squarrosulus</i> was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of <i>L. squarrosulus</i> was assessed using the brine shrimp lethality assay.</p><p><strong>Results: </strong>The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of <i>L. squarrosulus</i> with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of <i>L. squarrosulus</i> had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC<sub>50</sub> value of 22.28 ± 0.65 μg/mL than the CME, while that of the reference, <i>i.e.</i> orlistat was 2.28 ± 0.34 μg/mL. However, these extracts exhibited very low or no α-amylase inhibitory and cytotoxic activity at the tested concentrations.</p><p><strong>Conclusion: </strong>This study reveals that CME of <i>L. squarrosulus</i>, rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 6","pages":"642-648"},"PeriodicalIF":1.8000,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780580/pdf/TJPS-19-642.pdf","citationCount":"2","resultStr":"{\"title\":\"<i>Lentinus squarrosulus</i> Mont. Mushroom: Molecular Identification, In vitro Anti-Diabetic, Anti-Obesity, and Cytotoxicity Assessment.\",\"authors\":\"Oyindamola Olajumoke Abiodun, Adenike Martha Alege, Precious Ulonnam Ezurike, Abraham Nkumah, Oluwatosin Adelowo, Tolulope Aderinola Oke\",\"doi\":\"10.4274/tjps.galenos.2021.72798\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Mushrooms are fungi with nutritional and health benefits. <i>Lentinus squarrosulus</i> Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the <i>in vitro</i> anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom.</p><p><strong>Materials and methods: </strong><i>L. squarrosulus</i> was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of <i>L. squarrosulus</i> was assessed using the brine shrimp lethality assay.</p><p><strong>Results: </strong>The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of <i>L. squarrosulus</i> with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of <i>L. squarrosulus</i> had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC<sub>50</sub> value of 22.28 ± 0.65 μg/mL than the CME, while that of the reference, <i>i.e.</i> orlistat was 2.28 ± 0.34 μg/mL. However, these extracts exhibited very low or no α-amylase inhibitory and cytotoxic activity at the tested concentrations.</p><p><strong>Conclusion: </strong>This study reveals that CME of <i>L. squarrosulus</i>, rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities.</p>\",\"PeriodicalId\":23378,\"journal\":{\"name\":\"Turkish Journal of Pharmaceutical Sciences\",\"volume\":\"19 6\",\"pages\":\"642-648\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2022-12-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780580/pdf/TJPS-19-642.pdf\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkish Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4274/tjps.galenos.2021.72798\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4274/tjps.galenos.2021.72798","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Lentinus squarrosulus Mont. Mushroom: Molecular Identification, In vitro Anti-Diabetic, Anti-Obesity, and Cytotoxicity Assessment.
Objectives: Mushrooms are fungi with nutritional and health benefits. Lentinus squarrosulus Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the in vitro anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom.
Materials and methods: L. squarrosulus was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of L. squarrosulus was assessed using the brine shrimp lethality assay.
Results: The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of L. squarrosulus with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of L. squarrosulus had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC50 value of 22.28 ± 0.65 μg/mL than the CME, while that of the reference, i.e. orlistat was 2.28 ± 0.34 μg/mL. However, these extracts exhibited very low or no α-amylase inhibitory and cytotoxic activity at the tested concentrations.
Conclusion: This study reveals that CME of L. squarrosulus, rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities.