大香菇蘑菇:分子鉴定、体外抗糖尿病、抗肥胖和细胞毒性评估。

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Turkish Journal of Pharmaceutical Sciences Pub Date : 2022-12-21 DOI:10.4274/tjps.galenos.2021.72798
Oyindamola Olajumoke Abiodun, Adenike Martha Alege, Precious Ulonnam Ezurike, Abraham Nkumah, Oluwatosin Adelowo, Tolulope Aderinola Oke
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引用次数: 2

摘要

目的:蘑菇是一种具有营养和健康益处的真菌。大香菇是一种食用菌,在代谢性疾病的局部治疗中具有传统的用途和相关性。因此,本研究旨在评价香菇氯仿/甲醇提取物(CME)和水提取物(AE)的体外抗肥胖、抗糖尿病和细胞毒性潜力。材料与方法:利用分子生物学手段对小角蜱进行鉴定。依次获得CME和AE,然后进行α-淀粉酶、α-葡萄糖苷酶和脂肪酶抑制酶测定以及总酚含量(TPC)和类黄酮含量(TFC)测定。采用盐水对虾致死性试验,评价了虎耳草提取部位的细胞毒潜能。结果:分子鉴定结果表明,该菌内部转录的间隔序列与L. squarrosulus相当,相似度高,获得了唯一的加入号(KT120043.1)。与AE相比,白斑乳杆菌CME具有更高的TPC、TFC和α-葡萄糖苷酶抑制活性。与对照奥利司他的IC50值为2.28±0.34 μg/mL相比,香菇AE的IC50值为22.28±0.65 μg/mL,显示出更高的脂肪酶抑制电位。然而,这些提取物在测试浓度下表现出非常低或没有α-淀粉酶抑制和细胞毒活性。结论:本研究表明,白桦多酚富含多酚和黄酮类化合物,具有较强的α-葡萄糖苷酶和脂肪酶抑制活性。
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Lentinus squarrosulus Mont. Mushroom: Molecular Identification, In vitro Anti-Diabetic, Anti-Obesity, and Cytotoxicity Assessment.

Objectives: Mushrooms are fungi with nutritional and health benefits. Lentinus squarrosulus Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the in vitro anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom.

Materials and methods: L. squarrosulus was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of L. squarrosulus was assessed using the brine shrimp lethality assay.

Results: The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of L. squarrosulus with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of L. squarrosulus had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC50 value of 22.28 ± 0.65 μg/mL than the CME, while that of the reference, i.e. orlistat was 2.28 ± 0.34 μg/mL. However, these extracts exhibited very low or no α-amylase inhibitory and cytotoxic activity at the tested concentrations.

Conclusion: This study reveals that CME of L. squarrosulus, rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities.

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CiteScore
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