Dongdan Yuan, Lei Zheng*, Yong-Xin Liu, Hongguang Cheng, Aizhong Ding, Xiaomin Wang, Qiuyang Tan, Xue Wang, Yuzi Xing, En Xie, Haoming Wu, Shanyun Wang and Guibing Zhu,
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引用次数: 1
摘要
休眠的高原永久冻土融化通过湿地释放一氧化二氮(N2O);然而,高原湿地N2O的产生机制尚不清楚。利用15N-18O双示踪技术和宏基因组测序技术,对2020年夏季云贵和青藏高原湿地N2O生成机制进行了分析。所有16个沉积物样品(海拔1020-4601 m: 2.55±0.42-26.38±3.25 ng N g-1 d-1)均检测到N2O生产活性,并由硝化器反硝化(ND)促进。ND的关键功能基因(amoA、hao和nirK)属于完全氨氧化(comammox)细菌,ND的关键菌种为硝化亚硝基螺旋菌(Nitrospira nitrificans)。研究发现,高原湿地沉积物中氮化氮氧化菌(N. nitrificans)和氨氧化菌(Nitrosomonas europaea)共同产生N2O。此外,我们推断环境因子(海拔和总有机质(TOM))通过硝化氮菌影响合作模式,从而影响高原湿地沉积物中N2O的生产活动。我们的发现促进了对生物地球化学循环和全球气候变化中硝化物的机制理解。
Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments
The thawing of dormant plateau permafrost emits nitrous oxide (N2O) through wetlands; however, the N2O production mechanism in plateau wetlands is still unclear. Here, we used the 15N–18O double tracer technique and metagenomic sequencing to analyze the N2O production mechanism in the Yunnan–Kweichow and Qinghai–Tibet plateau wetlands during the summer of 2020. N2O production activity was detected in all 16 sediment samples (elevation 1020–4601 m: 2.55 ± 0.42–26.38 ± 3.25 ng N g–1 d–1) and was promoted by nitrifier denitrification (ND). The key functional genes of ND (amoA, hao, and nirK) belonged to complete ammonia oxidizing (comammox) bacteria, and the key ND species was the comammox bacterium Nitrospira nitrificans. We found that the comammox bacterial species N. nitrificans and the ammonia oxidizing bacterial (AOB) species Nitrosomonas europaea cooperate to produce N2O in the plateau wetland sediments. Furthermore, we inferred that environmental factors (elevation and total organic matter (TOM)) influence the cooperation pattern via N. nitrificans, thus affecting the N2O production activity in the plateau wetland sediments. Our findings advance the mechanistic understanding of nitrifiers in biogeochemical cycles and global climate change.
期刊介绍:
Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences.
Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.