细胞内核磁共振:为什么?如何?

IF 7.3 2区 化学 Q2 CHEMISTRY, PHYSICAL Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2022-10-01 DOI:10.1016/j.pnmrs.2022.04.002
Francois-Xavier Theillet , Enrico Luchinat
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引用次数: 17

摘要

早在1950年,核磁共振光谱学就开始应用于细胞和组织分析。我们试图在这里以一种说教的方式收集从核磁共振对活细胞的研究中产生的广泛多样的数据和想法。核磁共振光谱学覆盖了元素周期表的很大一部分,允许对所有生物体中各种各样的原子核进行无创检查。因此,它提供了细胞原子及其化学环境、动力学或相互作用的定量信息。我们将展示核磁共振研究已经产生了关于大量细胞分子和事件的宝贵知识,从水、盐、代谢物、细胞壁、蛋白质、核酸、药物和药物靶点,到pH值、氧化还原平衡和化学反应。在原子尺度上对如此众多的物体进行表征,从而形成了我们在多个层面上对细胞生命的心理表征,以及质谱法或显微镜等主要技术。核磁共振对细胞的研究伴随着核磁共振和代谢组学的发展,各个子领域蓬勃发展,创造了吸引人的名字:通量组学,食物组学,MRI和MRS(分别是活组织的成像和定位光谱),全细胞核磁共振,基于细胞配体的核磁共振,系统核磁共振,细胞结构生物学,细胞内核磁共振……所有这些都不是分开发展的,而是像编织的树干一样相互加强。因此,我们试图在这里提供一种对错综复杂的方法的大集合的分析说明,这些方法的集成已经并且将是它们成功的关键。我们提供了广泛的概述,首先是核磁共振在细胞环境中提供的各种类型的信息(“为什么”,面向广泛的读者),其次是所采用的核磁共振技术和设置(“如何”,我们讨论过去,现在和未来的方法)。每个小节都是作为一个历史选集构建的,展示了核磁共振波谱的内在特性及其发展如何构建了细胞现象的可访问知识。使用这种系统的方法,我们寻求i)使这篇综述能够被最广泛的受众所接受,ii)突出一些可能重新引起兴趣的早期技术。最后,我们简要讨论了在生物学综合研究的背景下可能存在的潜在和可取的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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In-cell NMR: Why and how?

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of data and ideas that emerged from NMR investigations on living cells. Covering a large proportion of the periodic table, NMR spectroscopy permits scrutiny of a great variety of atomic nuclei in all living organisms non-invasively. It has thus provided quantitative information on cellular atoms and their chemical environment, dynamics, or interactions. We will show that NMR studies have generated valuable knowledge on a vast array of cellular molecules and events, from water, salts, metabolites, cell walls, proteins, nucleic acids, drugs and drug targets, to pH, redox equilibria and chemical reactions. The characterization of such a multitude of objects at the atomic scale has thus shaped our mental representation of cellular life at multiple levels, together with major techniques like mass-spectrometry or microscopies.

NMR studies on cells has accompanied the developments of MRI and metabolomics, and various subfields have flourished, coined with appealing names: fluxomics, foodomics, MRI and MRS (i.e. imaging and localized spectroscopy of living tissues, respectively), whole-cell NMR, on-cell ligand-based NMR, systems NMR, cellular structural biology, in-cell NMR… All these have not grown separately, but rather by reinforcing each other like a braided trunk. Hence, we try here to provide an analytical account of a large ensemble of intricately linked approaches, whose integration has been and will be key to their success.

We present extensive overviews, firstly on the various types of information provided by NMR in a cellular environment (the “why”, oriented towards a broad readership), and secondly on the employed NMR techniques and setups (the “how”, where we discuss the past, current and future methods). Each subsection is constructed as a historical anthology, showing how the intrinsic properties of NMR spectroscopy and its developments structured the accessible knowledge on cellular phenomena. Using this systematic approach, we sought i) to make this review accessible to the broadest audience and ii) to highlight some early techniques that may find renewed interest. Finally, we present a brief discussion on what may be potential and desirable developments in the context of integrative studies in biology.

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来源期刊
CiteScore
14.30
自引率
8.20%
发文量
12
审稿时长
62 days
期刊介绍: Progress in Nuclear Magnetic Resonance Spectroscopy publishes review papers describing research related to the theory and application of NMR spectroscopy. This technique is widely applied in chemistry, physics, biochemistry and materials science, and also in many areas of biology and medicine. The journal publishes review articles covering applications in all of these and in related subjects, as well as in-depth treatments of the fundamental theory of and instrumental developments in NMR spectroscopy.
期刊最新文献
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