基于微流体和化学发光“接触”成像检测的即时细小病毒B19检测和基因分型

L. S. Dolci, M. Mirasoli, Angela Buragina, M. Guardigli, A. Roda, F. Bonvicini, Francesca Di Furio
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引用次数: 0

摘要

基于固定化寡核苷酸探针杂交和化学发光检测,研制了一种适用于细小病毒B19基因分型和DNA定量检测的诊断装置。微流控室直接与CCD传感器耦合在“接触”成像配置中,以获得适合即时护理应用的便携式设备。
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Point-of-care Parvovirus B19 detection and genotyping based on microfluidics and chemiluminescence “contact” imaging detection
A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.
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