Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954846
S. O’Keeffe, K. Bremer, U. Timm, D. McCarthy, G. Leen, E. Lewis
Advancements in optical technology have led to the development of a number of different sensor systems suitable for use in the biomedical industry, such as cardiovascular system monitors, lung/bladder pressure sensors and radiation dosimeters. Recent efforts in the Optical Fibre Sensors Research Centre at the University of Limerick have seen the application of all-optical sensors to these areas. This paper gives an overview to these activities and highlights the unique advantages optical sensors bring to this arena.
{"title":"Advances in all-optical sensors for biomedical monitoring","authors":"S. O’Keeffe, K. Bremer, U. Timm, D. McCarthy, G. Leen, E. Lewis","doi":"10.1109/IWBP.2011.5954846","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954846","url":null,"abstract":"Advancements in optical technology have led to the development of a number of different sensor systems suitable for use in the biomedical industry, such as cardiovascular system monitors, lung/bladder pressure sensors and radiation dosimeters. Recent efforts in the Optical Fibre Sensors Research Centre at the University of Limerick have seen the application of all-optical sensors to these areas. This paper gives an overview to these activities and highlights the unique advantages optical sensors bring to this arena.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126189153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954849
A. Candiani, M. Sozzi, E. Coscelli, F. Poli, A. Cucinotta, S. Selleri, R. Veneziano, R. Corradini, R. Marchelli, P. Childs, S. Pissadakis
We present a new approach for developing a label-free biosensor for detection of DNA. The biosensor based on a biofunctionalized Double Tilt Fiber Bragg Grating transduces the presence of DNA into a change of visibility of fringes of its interference spectrum. Real-time spectral measurement results are reported, showing that a 10nM DNA solution induces a 10% modulation of the corresponding fringes visibility.
{"title":"Double Tilted Fiber Bragg Grating for label-free DNA detection","authors":"A. Candiani, M. Sozzi, E. Coscelli, F. Poli, A. Cucinotta, S. Selleri, R. Veneziano, R. Corradini, R. Marchelli, P. Childs, S. Pissadakis","doi":"10.1109/IWBP.2011.5954849","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954849","url":null,"abstract":"We present a new approach for developing a label-free biosensor for detection of DNA. The biosensor based on a biofunctionalized Double Tilt Fiber Bragg Grating transduces the presence of DNA into a change of visibility of fringes of its interference spectrum. Real-time spectral measurement results are reported, showing that a 10nM DNA solution induces a 10% modulation of the corresponding fringes visibility.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"203 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122336807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954817
P. Taroni, A. Pifferi, G. Quarto, A. Torricelli, R. Cubeddu, L. Spinelli
Time domain diffuse optical mammography is performed at 4 to 7 wavelengths (635–1060 nm) for the detection and identification of breast lesions, and for the non-invasive assessment of breast density, a well-known risk factor for cancer development.
{"title":"Time domain diffuse optical imaging and spectroscopy of breast","authors":"P. Taroni, A. Pifferi, G. Quarto, A. Torricelli, R. Cubeddu, L. Spinelli","doi":"10.1109/IWBP.2011.5954817","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954817","url":null,"abstract":"Time domain diffuse optical mammography is performed at 4 to 7 wavelengths (635–1060 nm) for the detection and identification of breast lesions, and for the non-invasive assessment of breast density, a well-known risk factor for cancer development.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122203028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954851
F. Baldini, G. Ghini, A. Giannetti, F. Senesi, C. Trono
Monitoring pH for long periods, usually 24 h, in the stomach and in the esophagus may be essential in the diagnosis of gastro-esophageal diseases. The clinical range of interest is quite extended, between 1 to 8 pH units. Methyl red, after its covalent immobilization on controlled pore glass (CPG), is characterized by a working range which fits well with the clinical one. A novel probe, suitable for gastro-esophageal applications, was designed in order to optimize the performances of the colored CPG. This leads to a very simple probe configuration characterized by a very fast response.
{"title":"Novel fiber tip sensor potentially suitable for gastric pH measurement","authors":"F. Baldini, G. Ghini, A. Giannetti, F. Senesi, C. Trono","doi":"10.1109/IWBP.2011.5954851","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954851","url":null,"abstract":"Monitoring pH for long periods, usually 24 h, in the stomach and in the esophagus may be essential in the diagnosis of gastro-esophageal diseases. The clinical range of interest is quite extended, between 1 to 8 pH units. Methyl red, after its covalent immobilization on controlled pore glass (CPG), is characterized by a working range which fits well with the clinical one. A novel probe, suitable for gastro-esophageal applications, was designed in order to optimize the performances of the colored CPG. This leads to a very simple probe configuration characterized by a very fast response.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134330401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954857
Karthik Kumar, L. Isa, Andreas B. Dahlin Takumi, Erik Reimhul, E. Reimhult
We present nanopore and nanocone-containing nanopore arrays suited for membrance sensors. The unique potential advantages of asymmetric nanoparticle plasmonics are demonstrated for a simple setup for discriminatory, simultaneous sensing of different recognition events at two different hot pots. A novel particle lithography method based on liquid-liquid assembly to fabricate the sensor substrates is also presented.
{"title":"Fabrication and characterization of nanoscale structures for membrance sensing","authors":"Karthik Kumar, L. Isa, Andreas B. Dahlin Takumi, Erik Reimhul, E. Reimhult","doi":"10.1109/IWBP.2011.5954857","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954857","url":null,"abstract":"We present nanopore and nanocone-containing nanopore arrays suited for membrance sensors. The unique potential advantages of asymmetric nanoparticle plasmonics are demonstrated for a simple setup for discriminatory, simultaneous sensing of different recognition events at two different hot pots. A novel particle lithography method based on liquid-liquid assembly to fabricate the sensor substrates is also presented.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131079708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954847
M. Sozzi, E. Coscelli, F. Poli, A. Cucinotta, S. Selleri, R. Corradini, R. Marchelli, M. Konstantaki, S. Pissadakis
The feasibility of a DNA biosensor based on a long period grating inscribed in an optical fiber has been investigated. The sensor can perform a label-free detection, due to the functionalization of the optical fiber external surface with peptide nucleic acid probes matched with the DNA target strands. A wavelength shift of 1.2 nm for a 120 nM DNA solution has been measured. Fluorescence measurements have been also taken, in order to give a further proof of the fiber functionalization and hybridization.
{"title":"Long period grating-based fiber optic sensor for label-free DNA detection","authors":"M. Sozzi, E. Coscelli, F. Poli, A. Cucinotta, S. Selleri, R. Corradini, R. Marchelli, M. Konstantaki, S. Pissadakis","doi":"10.1109/IWBP.2011.5954847","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954847","url":null,"abstract":"The feasibility of a DNA biosensor based on a long period grating inscribed in an optical fiber has been investigated. The sensor can perform a label-free detection, due to the functionalization of the optical fiber external surface with peptide nucleic acid probes matched with the DNA target strands. A wavelength shift of 1.2 nm for a 120 nM DNA solution has been measured. Fluorescence measurements have been also taken, in order to give a further proof of the fiber functionalization and hybridization.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134500815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954843
M. L. Ermini, S. Scarano, M. Minunni
Improving the performances of a sensor is a prominent objective in developing innovative for clinical applications. Sensitivity is key features for a biosensor such applications. An improvement in sensitivity is reported when nanoparticles (NPs) are exploited for functionalizing the interacting surface. In this work an original nanostructure was investigated. Gold nanoparticles are immobilized on a gold surface of a Surface Plasmon Resonance imaging (SPRi) sensor. The surface structuring strategy was studied in its steps, for obtaining a reproducible immobilization of NPs on biochip gold surface. In order to improve our system, we modified SPRi chip with gold NPs attached on the surface trough a dithiol molecule. Further functionalization was achieved using thiolated DNA probes. The possibility to modify the biochip in an array format was really helpful for following the different steps of the procedure. The bioreceptors immobilization protocol was studied following the plasmon curves, confirming the presence of the nanostructure on the biochip surface.
{"title":"Surface nanostructuring for Surface Plasmon Resonance imaging","authors":"M. L. Ermini, S. Scarano, M. Minunni","doi":"10.1109/IWBP.2011.5954843","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954843","url":null,"abstract":"Improving the performances of a sensor is a prominent objective in developing innovative for clinical applications. Sensitivity is key features for a biosensor such applications. An improvement in sensitivity is reported when nanoparticles (NPs) are exploited for functionalizing the interacting surface. In this work an original nanostructure was investigated. Gold nanoparticles are immobilized on a gold surface of a Surface Plasmon Resonance imaging (SPRi) sensor. The surface structuring strategy was studied in its steps, for obtaining a reproducible immobilization of NPs on biochip gold surface. In order to improve our system, we modified SPRi chip with gold NPs attached on the surface trough a dithiol molecule. Further functionalization was achieved using thiolated DNA probes. The possibility to modify the biochip in an array format was really helpful for following the different steps of the procedure. The bioreceptors immobilization protocol was studied following the plasmon curves, confirming the presence of the nanostructure on the biochip surface.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128266084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954838
A. Candiani, M. Konstantaki, S. Pissadakis, C. Sterner, W. Margulis
A microstructured optical fibre Bragg grating modulator is implemented by infiltrating a short ferrofluid length into the fibre capillaries. Employing AC magnetic field amplitude modulations up to 5dB were archived.
{"title":"Microstructured optical fibre Bragg grating modulator employing an infiltrated ferrofluid","authors":"A. Candiani, M. Konstantaki, S. Pissadakis, C. Sterner, W. Margulis","doi":"10.1109/IWBP.2011.5954838","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954838","url":null,"abstract":"A microstructured optical fibre Bragg grating modulator is implemented by infiltrating a short ferrofluid length into the fibre capillaries. Employing AC magnetic field amplitude modulations up to 5dB were archived.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127032602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954803
G. Capelli, G. Giuliani
A self-mixing velocimeter system is demonstrated for the contactless measurements of the tangential speed of a rough surface. This system can be applied to the analysis of the eye movements in a range of angular velocity from 0°/s to 500°/s, also allowing a discrimination of the rotation direction, with a relative maximum error of 10–20%.
{"title":"Laser velocimeter for the measurement of eye movements","authors":"G. Capelli, G. Giuliani","doi":"10.1109/IWBP.2011.5954803","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954803","url":null,"abstract":"A self-mixing velocimeter system is demonstrated for the contactless measurements of the tangential speed of a rough surface. This system can be applied to the analysis of the eye movements in a range of angular velocity from 0°/s to 500°/s, also allowing a discrimination of the rotation direction, with a relative maximum error of 10–20%.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129139247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954820
S. Scarano, M. L. Ermini, Marcello Mascini, M. Minunni
Surface Plasmon Resonance imaging (SPRi) is at the forefront of optical sensing, allowing multi-analyte detection in real-time and without labeling. SPRi is applied to a variety of bio-interactions, from proteins to sensitive DNA detection. Despite the high number of recently appeared papers, very few deal with analyte detection in complex matrices. We here report SPRi affinity-based sensing with application to analytes detection in real matrices, i.e. proteins and DNA marker sequences for possible applications in different fields. In particular, we quantified Bovine IgG in cow milk avoiding matrix pre-treatments, and showing the simultaneous evaluation of different immobilization chemistries on a single biochip. We summarize results obtained in terms of perspectives for a real application of the biosensor in the field of food controls. About the detection of DNA sequences in complex matrices, we focused on DNA sequences in human transgenic cells for gene doping controls. Experimental results are briefly showed here in terms of advantages related to the application of SPRi technique to this emerging issue.
{"title":"Surface plasmon resonance imaging for affinity-based sensing: An analytical approach","authors":"S. Scarano, M. L. Ermini, Marcello Mascini, M. Minunni","doi":"10.1109/IWBP.2011.5954820","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954820","url":null,"abstract":"Surface Plasmon Resonance imaging (SPRi) is at the forefront of optical sensing, allowing multi-analyte detection in real-time and without labeling. SPRi is applied to a variety of bio-interactions, from proteins to sensitive DNA detection. Despite the high number of recently appeared papers, very few deal with analyte detection in complex matrices. We here report SPRi affinity-based sensing with application to analytes detection in real matrices, i.e. proteins and DNA marker sequences for possible applications in different fields. In particular, we quantified Bovine IgG in cow milk avoiding matrix pre-treatments, and showing the simultaneous evaluation of different immobilization chemistries on a single biochip. We summarize results obtained in terms of perspectives for a real application of the biosensor in the field of food controls. About the detection of DNA sequences in complex matrices, we focused on DNA sequences in human transgenic cells for gene doping controls. Experimental results are briefly showed here in terms of advantages related to the application of SPRi technique to this emerging issue.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125370253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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